通过盆栽实验,研究了EDTA、EGTA和柠檬酸(CA)3种螯合剂强化下新疆杨对干旱区农田土壤中Pb的吸收和富集特征。实验结果表明:与不投加螯合剂的对照相比,螯合剂的投加显著提高了土壤中水提态Pb的含量,其活化效果表现为EDTA〉EGTA〉CA,但螯合剂并未对杨树地上部分生物量产生显著的抑制作用。3种螯合剂均显著提高了杨树根中Pb的含量,其增幅达59%~87%,其中以9mmol·kg-1EDTA处理下增幅最大;3种螯合剂均未显著提高杨树树干中Pb的含量,但在EDTA的2个投加水平(3、9mmol·kg-1)作用下,杨树叶中Pb的含量和对照相比则显著增加,其增幅分别为125%和332%。Pb主要积累在杨树根部,3种螯合剂处理下杨树根中Pb的积累量占杨树总Pb积累量的比例由对照的88%增至94%~98%。总体而言,EDTA相比于EGTA和CA,更有助于杨树对土壤中Pb的吸收和积累。
To investigate the effects of ethylenediamine tetraacetic acid(EDTA),ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid(EGTA) and citric acid(CA) on lead(Pb) uptake and accumulation in parts of poplar(Populus bolleana Lauche),a pot experiment was designed and performed.Results showed that the addition of EDTA,EGTA and CA significantly increased water-extractable Pb concentration in the soil as EDTAEGTACA,but didn't reduce the aboveground biomass,as compared to the control.The concentration of Pb in the root significantly increased in response to the addition of EDTA,EGTA,and CA,with the increase ranged from 59%~87% and the highest increase occurred under the treatment of 9 mmol kg-1 EDTA.The concentration of Pb in the stem didn't change significantly in re-sponse to the addition of EDTA,EGTA,and CA compared with the control.The concentration of Pb in leaves,however,significantly in-creased in response to the addition of 3 mmol·kg-1 and 9 mmol·kg-1 EDTA with an increase of 125% and 332%,respectively.Additionally,Pb accumulated predominately in the root,the proportion of the amount of Pb in the root to the total amount of Pb in poplar increased from 88% of the control to 94%~98% after the addition of EDTA,EGTA,and CA.In general,EDTA is more efficient than EGTA and CA in stimu-lating the uptake and accumulation of Pb in parts of P.bolleana.