中文摘要：

【摘要】目的探讨尼古丁乙酰胆碱受体的CHRNA3-CHRNB4一CHRNA5基因簇多态性与焦炉TDNA损伤的联系。方法选择中国北方某焦化厂的309名工人作为研究对象，按照血浆中苯并（a）芘二氢二醇环氧化物（BPDE）一自蛋白加合物水平，将研究对象分为高暴露组（154人）和低暴露组（155人），使用健康体检调查表收集个人健康信息，采用碱性单细胞凝胶电泳技术评价淋巴细胞DNA损伤，基质辅助激光解吸电离飞行时间质谱技术检测基因多态性，使用Phasev2.1.1软件计算单体型对。结果高浓度多环芳烃暴露组T人外周血淋巴细胞彗星尾矩（OTM）值为1．23±1.05，低暴露组OTM值为O．80±1．07，两组比较，差异有统计学意义（P〈O．01）。在高暴露组中，CHRNA3-CHRNB4-CHRNA5基因簇的rs667282CC基因型携带者外周血淋巴细胞的OTM值（1．64±0．17）明显高于CT和CT＋TT基因型携带者（0．95±0．13和1．09±0．11），差异有统计学意义（P〈0．01）；rsl2910984GG基因型携带者外周血淋巴细胞的OTM值（1．60±0．17）明显高于AG和AG＋AA基因型携带者（0．92±0．13和1．07±0．10），差异有统计学意义（P〈O．01）。单体型对的分析结果显示，在高暴露组中，人群巾分布最广的TA／CG单体型对携带者OTM值最低（0．89±0．13），TA／TA和CG／CG单体型对携带者OTM值分别为1．35±0．17和1．64±0．17，TA厂11A和CG／CG单体型对携带者的OTM值明显高于TA／CG单体型对携带者，差异有统计学意义（P〈0．05）。在低暴露组中，没有发现该基因簇多态性与DNA损伤之间的关联。结论CHRNA3一CHRNB4一CHRNA5基因簇多态性与焦炉工DNA损伤程度有关联。暴露于高浓度多环芳烃时，携带rs667282位点CC基[大j型的个体DNA损伤程度较其他基因型携带者高；携带rsl2910984位点GG基冈型的个体DNA损伤程度较其他基因型携带者高。为进一步阐明CHRNA3

英文摘要：

Objective To investigate the relevance between single nueleotide polymorphisms （SNPs） in CHRNA3-CHRNB4-CHRNA5 gene cluster and DNA damage levels in the coke oven workers. Methods A total of 309 male subjects from a steel plant in Northern China were divided into high exposure group （154 workers） and low exposure group （ 155 workers） according to the concentrations of plasma benzoapyrene-r-7, t- 8, t-9, c-10-tetrahydotetrol-albumin （BPDE-AIb） adducts. DNA damage levels of peripheral blood lymphocytes were examined by comet assay. Allelie discrimination of SNPs in CHRNA3-CHRNB4-CHRNA5 gene cluster were detected using Matrix-assisted laser desorption/ionization mass spectrometry. Results The Olive tail mo- ment （OTM） （1.23±1.05） of peripheral blood lymphocytes in the high exposure group was significat~tly higher than that （0.80±1.07） in the low exposure group （P〈0.01）. In the high exposure group, OTM （1.64±0.17） of subjects with rs667282CC genotype was significantly higher than those （0.95±0.13 or 1.09±0.11） of subjects with rs667282CT genotype or rs667282TT＋CT genotype （P〈0.01）; OTM （1.600.17） of subjects with GG geno- type at rs12910984 site was significantly higher than those （0.92±0.13 or 1.07±0.10） of subjects with AG genotype or AG＋AA genotype （P〈O.01）; OTM （1.35±0.17 or 1.64±0.17） of the TA/TA or CG/CG diplotype carriers was significantly higher than that （0.89_＋0.13） of TA/CG diplotype carriers （P〈0.05）. However, the rele-vance between genotypes of these SNPs and the DNA damage levels was not found in the low exposure group. Conclusion The rs667282CC genotype and rs12910984GG genotype on CHRNA3-CHRNB4-CHRNA5 gene cluster are associated with increased DNA damage levels in the high PAHs exposure group.