以普通小麦川农19(N,7+8,2+12)和1BL/1RS易位系小麦川农18(1,7+9,2+12)F1为试材,用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳(sodium dodecyl sulphate—polyacrylamide gel electrophoresis,SDS-PAGE)法鉴定分析了杂交后代的高分子量谷蛋白亚基组成,发现其中1粒产生了2个亲本中都没有的高分子量谷蛋白亚基,其高分子量谷蛋白亚基组成为(1,X+7+8+9,2+12)。变异的亚基在SDS—PAGE电泳图谱上位于1Dx5和JBx7亚基之间,迁移率与1Bx6相似。统计变异株F2代HMW—GS的遗传规律,结果表明,Glu-Al位点上的亚基分离正常:而Glu—B1位点上亚基的分离以及Glu-A1与例Glu-B1位点之间的组合都不符合孟德尔遗传规律:变异亚基与IBy8亚基呈现共显性。最后对变异产生的机制进行了探讨。
The compositions of high-molecular-weight glutein subunits (HMW-GS) of F1 grains derived from wheat Chuannongl9 (N, 7 + 8,2 + 12) × Chuannong18 ( 1,7+ 9,2 + 12 ) were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS PAGE), and a HMW- GS variant was detected in one of the grains tested. The HMW-GS patterns of the variant seed were(1, x+ 7 + 8 + 9,2+ 12). SDS-PAGE analysis showed that the band of variant subunit was similar to 1Bx6, which located between 1Dx5 and 1Bx7. The HMW-GS subunits encoded by GlwB1 and the combination of Glu-Al and Glu-B1 loci deviated the Mendel's segregation ratios in Fe progenies. Furthermore,the variant subunit and the 1By8 were codominant. The forming mechanism of the variant HMW-GS was discussed at last.