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Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice
  • ISSN号:1673-1581
  • 期刊名称:《浙江大学学报:B卷英文版》
  • 时间:0
  • 分类:Q26[生物学—细胞生物学] S884.31[农业科学—特种经济动物饲养;农业科学—畜牧兽医]
  • 作者机构:[1]State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
  • 相关基金:Project supported by the National Natural Science Foundation of China (No. 30771399), the High-Tech Research and Development Program (863) of China (No. 2007AA10Z140) and the Research Fund for the Doctoral Program of Higher Education of the Ministry of Education of China (No. 20070335111)
中文摘要:

我们克隆并且描绘编码安培绑定蛋白质的米饭基因 OsBIABP1。OsBIABP1 的全身的 cDNA 长是 1912-bp 并且被预言编码 558-aa 蛋白质。OsBIABP1 包含一个典型安培绑定签名主题并且给安培绑定蛋白质家庭的成员显示出高类似。OsBIABP1 在米饭植物的茎,叶子和花被表示,但是没被表示或表示了在一很低级,在米饭根。OsBIABP1 的表示被一些防卫相关的信号分子导致,例如,水杨酸酸(SA ) 它调停, benzothiadiazole, jasmonic 酸(JA ) ,和 1-amino cyclopropane-1-carboxylic 酸 SA- 和 JA/ethylene (et ) 依赖防卫发信号小径分别地。而且, OsBIABP1 的表示被 Magnaporthe oryzae 的感染激活,并且导致的表示在在米饭和 M 之间的兼容相互作用比那在在不兼容的相互作用的致病的早阶段期间更快、更强壮。oryzae。我们的结果建议 OsBIABP1 可以是通过表明小径的 SA 或 JA/ET 涉及防卫反应的规定的防卫相关的安培绑定蛋白质。

英文摘要:

We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane- 1-carboxylic acid, which mediate SA- and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection ofMagnaporthe oryzae, and the induced expression is quicker and stronger during early stages ofpathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense response through SA and/or JA/ET signaling pathways.

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期刊信息
  • 《浙江大学学报:B卷英文版》
  • 中国科技核心期刊
  • 主管单位:
  • 主办单位:浙江大学
  • 主编:
  • 地址:杭州玉古路20号,浙江大学学报《英文版》编辑部
  • 邮编:310027
  • 邮箱:jzus@zju.edu.cn
  • 电话:0571-87952276 87952331
  • 国际标准刊号:ISSN:1673-1581
  • 国内统一刊号:ISSN:33-1356/Q
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  • 被引量:323