中文摘要：

用自制的玻璃微管（glass micropipette，GMP）冷冻猪卵母细胞。以FDA染色鉴定冷冻-解冻后卵母细胞存活力，结果表明，用常规细管程序化冷冻和GMP管玻璃化冷冻猪成熟（MⅡ期）卵母细胞时，冻后存活率分别为34．5％和63．3％（P〈0．05）；以玻璃化冷冻比较细管和GMP管对猪MⅡ期卵母细胞冷冻效果的影响，两组冻后存活率分别为45．0％和65．9％（P〈0．05）；用常规细管程序化和GMP管玻璃化冷冻猪GV期卵母细胞时，冻后存活率为30．0％对59．7％，有显著性差异（P〈0．05）研究表明，GMP法为一种有效的猪卵母细胞冷冻保存方法。

英文摘要：

The self-made glass micropipette （GMP） was used for cryopreservation of pig oocytes, obtaining good results. The post-thaw survival rate ofoocytes was assessed by fluorescein diacetate （FDA） staining. And the results showed that the programmed freezing method and GMP vitrification method respectively resulted in the post-thaw survival rate of 34.5% and 63.3%（P 〈0.05） in cryopreservation of porcine M Ⅱ stage oocytes. Comparing plastic straw with GMP in vitrification of porcine M Ⅱ stage oocytes, the survival rates of both groups were 45.0% and 65.9% （P 〈 0.05）, respectively. When GV-stage pig oocytes were cryopreserved by straw programmed freezing method and GMP vitrification method, they yielded survival rates of 30.0% and 59.7% respectively and there was significant difference between them （P 〈 0.05）. The present study shows that GMP method is an efficient freezing method for porcine oocyte cryopreservation.