中文摘要：

摘要：为实现低量豆粕（m_豆粕／m_豆油=10％）高效催化氧化大豆油合成氢过氧化豆油，对此氧化反应进行了单因素优化，并探讨了添加表面活性剂和引入超声辐射对豆油催化效率的影响。结果表明：豆粕催化豆油的优化条件为：在15℃下，0．05g豆粕溶于3，3mL pH6的磷酸盐缓冲液后，逐滴加入0．5g豆油和0．15mL乙醇，通氧反应3h后产率为12％。加入表面活性剂CTAB或β-环糊精后，反应产率呈现了不同程度的降低。超声辐射（100W，40Hz）提高了豆粕酶促催化氧化大豆油的效率，将反应的平衡时间减少为常规的1／3，将产率由12％提高到35％。

英文摘要：

The performance of low level soymeal （ m_soymeal/m_soybean oil = 10% ） in catalyzing the hydroperoxidation reaction using soybean oil as substrate was evaluated, and surfactants as well as ultrasonic wave were introduced to improve the lipoxygenation reaction yield. The optimal conditions for soybean oil were 0.5 g soybean oil in 3.3 mL phosphate buffer solution of pH6 with 0.15 mL ethanol as co-solvent reacting for 3 h at 15℃. Under those optimal conditions the yield reached 12%. The addition of surfactants such as CTAB and β-CD reduced the hydroperoxide yield. The employment of ultrasonic wave（ 100 W,40 Hz）during the aerobic catalysis reached equilibrium after 60 min with the yield of 35% ,which could both accelerate the enzymatic reaction and improve the reaction yield.