目的:研究青蒿酸对转基因西洋参(Panaxquinquefolium)冠瘿组织次生代谢物生产的影响。方法:青蒿酸西洋参冠瘿组织培养体系中,共培养2d,终止反应。产物用色谱法进行分离,根据其理化性质及波谱数据对分离所得的化合物进行结构鉴定。结果:分离并鉴定三个羟基十八碳烯酸类化合物,分别为:9,12,13-三羟基-10-十八碳烯酸(1)、11,12,13-三羟基-9-十八碳烯酸(2)和11-羟基-12,13-环氧-9-八碳烯酸(3)。结论:青蒿酸作为一种植物激素可诱导西洋参冠瘿组织培养体系中13-脂氧合酶的表达。
Objective: To investigate the effect of artermisinic acid on the secondary metabolites production of Panax quinquefolium crown galls. Methods: Artemisinic acid was added into the suspended cells of Panax quinquefolium crown galls and co-culture for two days. Products were isolated with chromatographic method. Results : Three hydroxyl octadecenoic acids [ 9,12,13-trihydroxy-10- octadecenoic acid ( 1 ), 11,12,13-trihydroxy-9-oetadeeenoie acid (2) and 11-hydroxy-12,13-epoxy-9-oetadeeenoic acid (3) ] were i- solated from crown galls of Panax quinquefolium. Conclusion : Artermisinic acid as one of the new type of phytohormones that might in- duce the production of 13-1ipoxygenases in crown galls of Panax quinquefolium.