从水产养殖污泥中分离筛选了一株优良的亚硝酸盐降解菌AQ-3,其在1.0×107 cfu/mL时对50mg/L亚硝酸盐的去除率高达99.47%。通过细菌自动鉴定系统(API ID32GN系统)以及16SrDNA系统发育分析,菌株AQ-3被鉴定为鲍曼氏不动杆菌(Acinetobacter baumannii)(JF751054),其16SrDNA序列与基因库中不动杆菌属菌株的16SrDNA序列有99%的同源性,而且与鲍曼氏不动杆菌菌株14(FJ907197)的亲缘关系最近。此外,菌株AQ-3同时含有nirS基因、nirK基因和norB基因,这些基因对AQ-3高效降解亚硝酸盐具有重要的意义。
A potential nitrite degrading bacterium AQ-3 was isolated and screened from the aquaculture sediment.The nitrite removal rate of AQ-3 was detected as 99.47%.Strain AQ-3 was identified as Acinetobacter baumannii(JF751054) through API ID32GN bacterial identification system and 16S rDNA sequence analysis.Its 16S rDNA sequence had homology of 99% with those of Acinetobacter sp.submitted to GenBank,and showed the closest relative to Acinetobacter baumannii strain 14(FJ907197).In addition,nirS gene、nirK gene and norB gene all existed in strain AQ-3.This study proved strain AQ-3 was a potential nitrite degrading bacterium,and enriched the microbial resources of nitrite degrading bacterium.