为探究NaCl胁迫下Si对甘草抗逆生理特性的影响,通过盆栽试验,研究不同浓度Si对甘草叶片抗氧化酶活性(SOD、POD、CAT)、MDA含量、质膜相对透性以及可溶性蛋白和脯氨酸含量的影响。结果表明,Si2(0.2g·kg-1)处理为最适浓度,可显著提高甘草叶片SOD、POD、CAT活性,与对照(Si0)相比,这3种酶活性在Si2处理下分别增加37.44%、26.98%、35.16%;Si3(0.6 g·kg-1)处理显著降低膜脂过氧化物MDA含量;增加质膜相对透性、可溶性蛋白含量;降低脯氨酸含量,MDA含量降低35.63%;质膜相对透性增加17.69%;可溶性蛋白含量增加36.62%;脯氨酸含量降低38.15%。由此可见,Si可以通过提高抗氧化酶活性来减轻活性氧及自由基对甘草叶片的毒害作用,即加Si可改善甘草在NaCl胁迫中的抗逆特性,缓解了NaCl胁迫对甘草的伤害。
The experiment was carried out to study the effects of silicon on antioxidant enzymes(SOD, POD, and CAT) and osmolytes content of licorice leaves under NaCl stress by pot experiment. The results showed that the concen-tration of 0.2 g ·kg-1 silicon had the strongest effect increasing the activities of SOD, POD, and CAT, by 37.44% , 26. 98% , and 35.16% compared with the control, respectively. For the concentration of 0.6 g·k g -1, MDA was decreased by 35.63%, proline content by 38.15 % , while the relative membrane permeability was increased by 17.69%, and the soluble protein content by 36.62% . In conclusion, silicon treatment could alleviate the damage degree of NaCl stress by promoting the antioxidant enzymes activities in licorice leaves.