中文摘要：

目的：观察川芎嗪腹腔注射治疗大鼠偏头痛的效果,并探讨其可能机制。方法雄性SD大鼠30只,随机分为3组各10只,A组大鼠制模前腹腔注射盐酸川芎嗪注射液,B、C组腹腔注射等量生理盐水,连续7 d。 A、B组末次给药30 min后左肩部皮下注射硝酸甘油注射剂以制备偏头痛模型,C组同期左肩部皮下注射生理盐水0.5 mL。观察各组造模后各时间段爬笼、挠头次数,采用免疫荧光法、Western blotting法检测各组三叉神经颈髓复合体（TCC）中瞬时受体电位香草酸亚型1（TRPV1）蛋白,实时荧光定量PCR技术检测TRPV1 mRNA。结果与B组比较,A、C组制模后各时段大鼠爬笼次数、挠头次数均减少（除A、B组制模后0~30 min大鼠挠头次数比较外,P均＜0.05）。各组大鼠TCC内均有TRPV1不同程度阳性表达,B组表达亮度较A、C组明显。与B组比较,A、C组的TRPV1蛋白、mRNA相对表达量减少（P均＜0.01）。结论川芎嗪腹腔注射对偏头痛大鼠具有一定的治疗作用,其作用机制可能与抑制TCC中TRPV1表达有关。

英文摘要：

Objective To explore the effect and mechanism of intraperitoneal injection of tetramethylpyrazine for the treatment of migraine in rats. Methods Thirty Sprague-Dawle （ SD） rats were randomly divided into three groups of 10 in each group：groups A, B and C. Rats in the group A were given intraperitoneal injection of ligustrazine hydrochloride injec-tion before modeling, groups B and C were given intraperitoneal injection of the same volume of normal saline for 7 days. Mi-graine rat model was established by subcutaneously injection of nitroglycerin after 30 min of the last delivery in the groups A and B, and group C with subcutaneous injection of 0. 5 mL normal saline. We observed the frequency of scratching head and climbing cage of each rat. The expression of transient receptor potential cation channel subfamily V member 1 （TRPV1） pro-tein in rat trigeminocervical complex （ TCC） was detected with Western blotting, and the expression of TRPV1 mRNA was de-tected by real-time fluorescence quantificative PCR. Results Compared with group B, the frequency of scratching head and climbing cage in each period was significantly decreased in the groups A and C （ except the frequency of scratching head be-tween groups A and B in the 0-30 min after molding, statistical difference was found, all P〈0. 05）. Different levels of posi-tive expression of TRPV1 receptors in TCC of each group were observed by immunofluorescence. Compared with groups A and C, the expression intensity was higher in the group B, there was no statistical difference between group A and B. Compared with group B, the protein and mRNA expression of TRPV1 in TCC of group A and C were significantly decreased （ all P〈0. 01）. Conclusion Intraperitoneal injection of tetramethylpyrazine on migraine rats has a certain therapeutic effect, and the mechanism may be related to the inhibition of TRPV1 expression in trigeminal sensory neurons.