【目的】研究水杨酸对镉胁迫下葡萄根系特性的影响,探讨缓解葡萄镉伤害的途径。【方法】以‘泽香’葡萄扦插苗为试材,在水培条件下,研究水杨酸预处理对氯化镉胁迫下葡萄根系活力、质膜H^+-ATPase和Ca^+-ATPase活性以及活性氧和一氧化氮(NO)生成的影响。【结果】0.10mmol·L^-1氯化镉能够提高根系活力和质膜Ca^2+-ATPase活性;1.0mmol·L^-1氯化镉明显促进根系超氧阴离子(Oi)、H2O2和NO的生成,显著抑制根系活力及质膜W-ATPase和Ca^2+-ATPase活性。50μmol·L^-1。水杨酸显著降低1.0mmol·L^-1氯化镉处理下根系O2、H2O2和NO的生成,阻止根系活力及质膜H*-ATPase和Ca^2+-ATPase活性下降,而随着浓度升高至200μmol·L^-1,水杨酸的这种作用减弱。【结论】水杨酸缓解葡萄根系镉伤害的适宜浓度为50μmol·L^-1;在该浓度下,水杨酸通过降低镉胁迫下自由基的产生而减轻镉对葡萄根系活力和质膜ATPase的损伤。
[Objective] To explore the effect of salicylic acid (SA) on cadmium stress and the pathway of alleviating cadmium damage to grapes roots. [Method] The experiment was carried out with the cutting seedlings of Zexiang grape pretreated by salicylic acid under CdC12 stress via hydroponics. The activity of root, the activity of H^+-ATPase and Ca^2+-ATPase in plasma membrane (PM), and tbe formation of reactive oxygen species and nitric oxide (NO) were determine. [Result] The 0.01 mmol·L-1 CdCl2 increased the activity of root and PM Ca^2+-ATPase. 1.0 mmol·L^-1 CdCl2 promoted the formation of O2, H2O2 and NO, and inhibited significantly root activity and the activity of H^+-ATPase and Ca^2+-ATPase in PM. Pretreatment of SA at 50 μmol·L^-1 greatly decreased the formation of O2, H2O2 and NO, which retarded the declining of root activity and the activity of Hμ+-ATPase and Caμ2+-ATPase in PM under 1.0 mmol·L-1 CdCl2 treatment. This effect of SA on roots decreased when SA concentration was raised to 200 μmol·L^-1. [Conclusion] It was found that SA at 50 μmol·L^-1 is the optimal concentration for Alleviation of the damage of cadmium on grape roots, and at this concentration, SA mitigated the injurious effects of cadmium on root activity and PM ATPase in roots of grape by inhibiting the formation of free radicals.