中文摘要：

选取大肠埃希氏菌（Escherichiacoli）为示踪菌种，研究了紫外消毒和次氯酸钠消毒的灭活效果（用对数灭活率来衡量），进行了实时荧光定量聚合酶链式反应（PCR）检测，同时考察了浊度、Fe3＋浓度、有机物对紫外消毒的灭活效果影响，以及pH、氨氮浓度对次氯酸钠消毒的灭活效果影响。结果表明：（1）紫外消毒和次氯酸钠消毒对大肠埃希氏菌均有较好的灭活效果。紫外辐射剂量为15mJ／cm2时即可达到4．55的对数灭活率；次氯酸钠投加量为2．5mg／L，消毒时间30min即可i00％灭活。（2）当紫外辐射剂量为15mJ／cm2时，浊度、Fe3＋浓度增加或投加腐殖酸均可使紫外消毒的灭活效果变差。（3）pH升高或者氨氮浓度增大均会导致次氯酸钠消毒的灭活效果变差。

英文摘要：

Escherichia coli were selected as bacteria tracer to evaluate the disinfection efficiency of UV and sodium hypochlorite （NaC10）. The disinfection efficiency was evaluated by logarithmic inactivated rate of Escherichia coli ,which was obtained by the real-time fluorescence quantitative PCR detection method. The effect of the turbidity, Fe3＋ concentration,organic matter on UV disinfection,as well as pH,ammonia nitrogen concentration on sodium hypochlorite disinfection were also investigated. The result indicated that both UV disinfection and sodium hypochlorite disinfection showed perfect Escherichia coli inactivation efficiency. The inactivation rate of 4.55 could be achieved under UV dose of 15 mJ/cm2. Escherichia coli were 100% inactivated after exposure in 2.5 mg/L of sodium hypochlo- rite for 30 min. Higher turbidity and Fe3＋ concentration or the existence of humic acid could decrease the Escherichia coli inactivation rate of UV disinfection. For sodium hypoehlorite disinfection, the Escherichia coli inactivation effi- ciency was relative poor under higher pH or ammonia nitrogen concentration.