中文摘要：

目的探讨大鼠脊髓源性少突胶质前体细胞在体外条件下的增殖及分化等生物学特性,为进一步研究脊髓脱髓鞘疾病的髓鞘再生奠定基础。方法采用免疫吸附法原代培养大鼠脊髓源性少突胶质前体细胞,并用免疫荧光染色鉴定其表面标志物A2B5及NG2;采用Alarm blue法检测其在体外的增殖能力,并绘制生长曲线;诱导原代培养的少突胶质前体细胞向成熟少突胶质细胞分化,并用免疫荧光染色鉴定成熟少突胶质细胞表面标志物MBP及GalC的表达情况。结果免疫吸附法可以对大鼠脊髓组织中的少突胶质前体细胞进行有效的分离纯化,分离获得的少突胶质前体细胞A2B5及NG2表达呈阳性,Alarm blue法检测结果显示少突胶质前体细胞在体外具有良好的增殖能力,诱导分化实验证实少突胶质前体细胞可以分化为成熟少突胶质细胞并且MBP及GalC表达呈阳性。结论采用免疫吸附法原代培养的大鼠脊髓源性少突胶质前体细胞在体外具有良好的增殖能力以及分化为成熟少突胶质细胞的能力。

英文摘要：

Objective To study the characteristics of proliferation and differentiation of oligodendrocyte precursor cells（OPCs）derived from the spinal cord of rats in vitro.Methods OPCs were separated from the spinal cord of neonatal rats and then purified with immunopanning method.The purified OPCs were identified by immunofluorescence staining with NG2 and A2B5.The characteristic of proliferation of OPCs in vitro was studied by alarm blue assay.The purified OPCs were induced to differentiate into mature oligodendrocytes and observed by immunofluorescence staining with MBP and GalC.Results OPCs derived from the spinal cord of rats could be purified by using immunopanning method.The purified OPCs were positively stained with NG2 and A2B5,and retained the proliferative capacity in vitro.Moreover,after the differentiation culture,the purified OPCs derived from the spinal cord of rats could differentiate into mature oligodendrocytes which expressed MBP and GalC.Conclusion The OPCs derived from the spinal cord of rats can be effectively purified by using immunopanning method,and the purified OPCs have good capacity of proliferation and differentiation in vitro.