目的:探讨NaCl胁迫对甘草生长、生理及有效成分含量的影响。方法:以药用植物甘草为材料,采用水培方式培养,以含有不同质量浓度NaCl的营养液对其进行不同强度的盐胁迫处理,分别于胁迫后10,20,30,40 d动态取样,测定甘草的生长、生理指标和甘草酸、甘草苷含量。结果:处理20,30,40 d时,0.6%和0.8%处理组的甘草植株株高、甘草根鲜重显著低于对照组;处理30,40 d时,0.6%和0.8%处理组的甘草干重均显著低于对照组;处理20 d,0.6%和0.8%处理组的甘草叶片SOD酶活性和叶绿体色素的含量均极显著高于对照组;处理30 d,0.6%和0.8%NaCl处理的甘草中甘草酸极显著高于对照组,处理20 d和30 d,0.6%,0.8%NaCl处理的甘草苷含量极显著高于对照组。结论:NaCl胁迫下,甘草生长受到了抑制,并通过提高体内的抗氧化酶活性、降低叶绿体色素含量和促进次生代谢产物积累,来提高自身耐盐性,同时也提高了药材的质量。
Objective:To study the effects of NaCl stress on growth,physiological index and content of effective composition of Glycyrrhiza uralensis.Method:Medicinal plant Gomposition uralensis was taken as the material.The rhizomes were grown in nutrient solution,and stressed with different concentration of NaCl.Respectively sampling after treatment of 10,20,30,40 days,liquorice growth,physiological indexes and glycyrrhizic acid and liquorice contents were determined.Result:On the treatment of 20,30,40 days,the height and the root fresh weight of 0.6% and 0.8% treatment groups were decreased as compared with CK significantly,while on the treatment of 30 and 40 days,the dry weight of 0.6% and 0.8% treatment groups were decreased as compared with control group significantly.On the treatment of 20 days,the SOD acticity and the the chloroplast pigment content of 0.6% and 0.8% treatment groups were extremely significant higher than those in the control group.And on the treatment of 30 days,the content of the glycyrrhizic acid of 0.6% and 0.8% treatment groups were increased as compared with control group significantly.While on the 20 and 30 days,the content of liquorice of 0.6% and 0.8% treatment groups were increased as compared with control group significantly.Conclusion:NaCl stress inhibited the growth of G.uralensis,and through improving the body of antioxidant enzymes activity,reducing the chloroplast pigment contents and promoting secondary metabolites accumulation,it improved its salt tolerance and the quality of G.uralensis.