中文摘要：

超临界流体萃取（SFE）技术已经被广泛用于天然产物中有效成分的提取，它易于实现与高效液相色谱、气相色谱和超临界流体色谱等分析仪器的在线联用，其中SFE-HPLC在线联用系统可以将萃取物完全转移到分析柱，使得检测灵敏度大大提高，所需样品量减小，分析时间缩短，而且能够避免样品污染、变质，尤其适合对光或空气敏感型的化合物、珍贵样品和痕量样品的萃取分离分析。本文在前期研究的基础上，发展了采用自动十通阀／并行捕集柱接口构建了SFE和HPLC在线联用的新装置，并利用该系统对灵芝子实体的超临界萃取过程进行实时监测。

英文摘要：

A novel on-line parallel-trap supercritical fluid extraction （SFE）-high performance liquid chromatography（ HPLC ） system was established. The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercfitical carbon dioxide, and HPLC was used to analyze the transferred sample. The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum. The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined, which accorded with the result obtained with off-line extraction. Using this system, the whole process can be monitored and the optimum extraction time can be determined with single run. In addition, it has many other advantages, such as low sample loading, high sensitivity and accuracy, short analysis time, avoiding sample pollution and easily operation etc. The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.