中文摘要：

采用Wistar大鼠骨髓单核细胞及RAW264.7细胞在体外诱导分化为破骨细胞（OC）,通过抗酒石酸酸性磷酸酶（TRAP）染色鉴定破骨细胞,荧光显微镜观察破骨细胞骨架F-actin,扫描电子显微镜检测骨吸收陷窝,比较两种方法诱导形成的破骨细胞细胞骨架F-actin的差异。结果表明：大鼠骨髓单核细胞诱导形成的破骨细胞数量及骨吸收活性均极显著高于RAW264.7细胞（P〈0.01）;两种方法诱导形成的破骨细胞骨架均具有典型的F-actin环结构。证明大鼠骨髓单核细胞及RAW264.7细胞均可诱导形成破骨细胞,两者破骨细胞骨架F-actin环结构无显著差异,但大鼠骨髓单核细胞诱导形成的破骨细胞数量更多,骨吸收活性更强。

英文摘要：

In this paper,the diversity of cytoskeleton F-actin in OC cultured by two different methods,which induced from Wistar rat bone marrow mononuclear cell and RAW264.7cell in vitro,was compared.Osteoclast differentiation cytoskeleton and activation were estimated via TRAP staining assay,TRITC-conjugated phalloidin staining,resorption activity analysis.The results showed that,the number of TRAP-positive cells and the area of bone resorption pits induced from rat bone marrow mononuclear cells was significantly higher than that induced from RAW264.7cell（P〈0.01）.However,there was no difference of F-actin ring between osteocalsts induced from rat bone marrow mononuclear cells and from RAW264.7cell.The results demonstrated that,osteoclasts with typical characteristic can form in the two methods with no significant difference on cytoskeleton,the cell number was more higher and the bone resorption activity was more powerful with OC induced from bone marrow mononuclear cells.