中文摘要：

为确诊锦州某蜂场蜜蜂大量死亡病因,本试验采用反转录-聚合酶链反应（RT-PCR）方法,收集病死蜜蜂提取总RNA,进行反转录,获得c DNA。然后,以c DNA为模板,利用检测常见蜜蜂病毒的引物进行PCR扩增。结果表明,从蜜蜂病料中扩增出编码蜜蜂残翅病毒Helicase蛋白酶的基因片段,大小约702 bp,将分离毒株命名为DWV-JZ。测序结果通过BLAST比对以及与Gen Bank中获得的7个序列进行遗传进化分析,证实DWV-JZ与Gen Bank中的DWV-JL1（KP096414.1）同源性达到95%,亲缘关系最近。

英文摘要：

To diagnose the cause of death of a large number of bee in Jinzhou a apiary. In this study, total RNA was extracted from dead bees, and eDNA were obtained by reverse-transcriptase PCR （RT-PCR） method. Then, using eDNA as a template, PeR amplification was performed using prim- ers for detecting common honeybee viruses. The results show, a gene fragment about 702 bp encoding deformed wing virus helicase protease was amplified from the bee material, the separation of the virus named DWV-JZ. Sequencing results were analyzed by BLAST and proceeded phylogenetie analysis with seven reference sequences obtained in GenBank. It was confirmed that DWV-JZ had 95% ho- mology with DWV-JLl（KP096414.1） in GenBank and had the closest genetic relationship.