中文摘要：

参照CLSI推荐的微量肉汤稀释法检测了15种抗菌药物对91株鸡源大肠杆菌的最小抑菌浓度（MIC）,采用PCR方法检测其整合子-基因盒的分布情况,并用脉冲场凝胶电泳（PFGE）分析大肠杆菌的同源性。结果显示,91株大肠杆菌对15种抗菌药物的耐药率为3.3%-98.9%,且全部为四重耐药以上,多重耐药率为100%。91株大肠杆菌全部携带Ⅰ型整合子,其中70株带有编码耐氨基糖苷类和磺胺类药物的基因盒,分别是dfr A1＋tnp A IS26＋aad A1（45/70）,dfr A12＋aad A2（16/70）和dfr A1＋aad A1（9/70）,未检出Ⅱ型整合子。PFGE分型图谱显示,91株大肠杆菌共产生63个谱型,菌株之间的相似系数为30%-100%,31种谱型的同源性高达90%以上。其中41种带型只包含1株菌,其余22种带型包含菌株数为2-4株。结果表明,安徽合肥地区鸡源大肠杆菌对抗菌药物的耐药性较高,Ⅰ型整合子分布广泛,菌株基因分布呈多态性但同源性较高。

英文摘要：

The broth micro-dilution method was used to detect the antimicrobial sensitivity of 15 antibiotics against the 91 E.coli isolates.A PCR was used to detect the prevalence of integrons and gene cassettes in these isolates.And genotyping was carried out by pulsed field gel electrophoresis（PFGE）.The results showed that drug res-istance rates of 91 E.coli isolates to 15 antibiotics ranged from 3.3% to 98.9%.All stains were resistant to four or more antimicrobials and the multi-drug resistance rate was 100%.And all isolates harbored class Ⅰ integrons in which 70 contained gene cassettes（45 dfr A1-tnp A IS26-aad A1,16 dfr A12-aad A2,and 9 dfr A1-aad A1）.Class Ⅱ integrons were not detected.The PFGE genotyping of 91 isolates yielded 63 PFGE types,the similarity between strains was from 30% to 100%. Homology of 31 PFGE types were more than 90%. Out of 63 PFGE types, 41 types contain 1strain,22 types contain 2 to 4 strains.The results indicated that the resistant spectrum of E.coli was wide and serious.Class Ⅰ integrons were wide- spread and gene was polymorphism but high homology in Hefei dis trict of Anhui Province.