中文摘要：

目的建立营养性肥胖大鼠动物模型。方法雄性SD大鼠50只随机分成高脂饮食组和普通饮食组，于喂养第12周时测大鼠体重、血脂、空腹胰岛素、葡萄糖耐量、胰岛素耐量，于第16周留尿测定微量白蛋白和肌酐，留取肾周和附睾脂肪称重，分离大鼠胰岛进行体外培养，测定胰岛及单个细胞直径。结果与对照组相比，高脂组大鼠体重、肾周及附睾脂肪含量、血脂、空腹胰岛素水平以及尿白蛋白的排出均明显升高，存在糖耐量减低、胰岛素抵抗。高脂组大鼠中直径大于200μm的大胰岛比例增多，胰岛细胞数目增多。结论建立的肥胖大鼠模型较接近成年人肥胖症的病理生理改变，可作为肥胖及相关疾病的研究平台。

英文摘要：

Objective To establish rat model with nutritional obesity. Methods Fifty male SD rats were randomly divided into two groups and maintained for 16 weeks on high fat diet and normal diet respectively. Body weight, serum lipids, fasting insulin, intraperitoneal glucose tolerance test （IPGTT） and insulin tolerance test （ITT） were measured after 12 weeks in the two groups. Urinary albumin/ereatinine ratio （ACR）, perirenal fat and inguinal fat were measured after 16 weeks, as well as islets of these rats were isolated for comparing their diameter. Results High-fat diet resulted in a significant increase in body weight, serum lipids, fasting insulin, ACR, glucose intolerance, insulin resistance, as well as the content of perirenal fat and inguinal fat. Both the proportion of diameter≥200μm of islets and the number of pancreatic cells from rats with obesity were significantly higher than those of the control group. Conclusion Established rat model with obesity which are closer to the pathophysiological changes of adult obesity may provide the research platform for obesity and related diseases.