中文摘要：

采用重氮化法将磺胺二甲嘧啶（Sulfadimidine,SM2）与牛血清白蛋白（Bovine serum albumin,BSA）和卵清蛋白（Ovalbumin,OVA）交联合成人工抗原SM2-BSA与SM2-OVA,并研究了SM2重氮盐的稳定性,以确保偶联反应顺利进行.通过紫外扫描、高效毛细管电泳检测合成效果,用免疫原（SM2-BSA）免疫BALB/c小鼠,检测抗血清.结果表明,SM2-BSA和SM2-OVA均偶联成功.

英文摘要：

The hapten sulfadimidine（SM2） was linked to carrier protein bovine serum albumin（BSA） or ovalbumin（OVA） to generate artificial antigens,SM2-BSA and SM2-OVA by diazotization.To ensure the success of coupling reaction,the stability of SM2 diazonium salts was studied.The artificial antigens were identified by ultraviolet absorption and high performance capillary electrophoresis.SM2-BSA was further used to immunize BALB/c mice,and the generation of antiserum was detected.The results showed that the artificial antigens had been synthesized successfully.