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中文摘要：
为原核表达鸭坦布苏病毒（Duck tembusu virus，DTMUV）NSl重组蛋白，本研究通过RT．PCR方法扩增NSl基因，将其亚克隆至pET32a（＋）载体中构建重组表达质粒pET32a．NSl。将其转化大肠杆菌Rosetta中，经IPTG诱导表达了约59ku的重组蛋白。Westernblot分析表明，该重组蛋白可以与DTMUV阳性血清发生特异性反应，表明其具有良好的反应原性。
英文摘要：
To express the NS1 of duck-origin Tembusu virus, the NS1 gene of DTMUV was amplified by RT-PCR and was cloned into pET32a（＋） for expression in E.coli. The recombinant protein was highly expressed in a molecular weight of 59 ku. Western blot showed that the recombinant protein was reacted positively against the DTMUV antibodies. The results provide important basis for establishment of diagnostic and seroepidemiological monitoring method for DTMUV infection in ducks.
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