中文摘要：

从安徽省合肥地区4个养鸡场分离鉴定184株大肠杆菌,用PCR方法对Ⅰ型整合子及其基因盒的流行分布进行研究,用微量肉汤稀释法测定分离菌株对16种抗菌药物的最小抑菌浓度。PCR结果显示,184株鸡源大肠杆菌中49.45%（91/184）检出Ⅰ型整合子。91株整合子阳性大肠杆菌中,70株携带耐氨基糖苷和磺胺类药物的基因盒,分别是dfrA1＋tnpAIS26＋aadA1（45/70）基因盒、dfrA12＋aadA2（16/70）基因盒和dfrA1＋aadA1（9/70）基因盒,21株不携带基因盒。药敏实验结果显示,184株大肠杆菌对16种抗菌药物的耐药率为2.17%-97.83%,91株整合子阳性大肠杆菌对3种及3种以上药物的耐药率为97.8%;与整合子阴性菌株相比耐药率有显著差异,表明大肠杆菌的多重耐药性与整合子阳性检出率相关。

英文摘要：

A total of 184 isolates ofEscherichia coli were identified from four chicken farms in Hefei districtof Anhui Province. Prevalence of class I integrons and gene cassettes was detected using PCR. Susceptibilities ofthe E. coli isolates to 16 antimicrobials were determined using broth micro-dilution method. The PCR resultsshowed that 91 of 184 （49.45%） isolates harbored class I integrons in which 70 contained gene cassettes （45dfrAl-tnpAIS26-aadA1, 16 dfrA2-aadA, and 9 dfrAl-aadA1）. Twenty one isolates did not carry gene cassettes.Susceptibility test showed that drug resistance rates of 184 E. coli isolates to 16 antimicrobials ranged from 2.17%to 97.83%. The rate of drug resistance to three and more antimicrobials was 97.8%. There was a significant dif-ference in drug resistance between isolates with and without integrons, indicating that multiple drug resistance ofE. coli is correlated with the presence of integrons.