中文摘要：

对186份大豆种质资源进行了成株和种粒斑驳抗性鉴定，并利用与成株抗性及种粒斑驳抗性分别相关的SSR标记验证抗病毒分子辅助选择的可行性。结果表明：接种SMV1，选出成株和种粒双抗种质38份，成株抗病种质149份，种粒抗病种质45份，成株和种粒双感种质26份。利用与成株抗性相关的SSR标记Sat_229、Sat_317、Satt335、Satt160、Satt516、Sat_309进行检测，抗病毒资源筛选的准确率分别达到68．9％、74．3％、71．1％、69．8％、77．4％和68．2％。利用与种粒斑驳抗性相关的SSR标记Sat_297、Sat_229、Sat_317、Satt335、Set_188、Satt160、Satt516、Sat_133进行检测，Sat_317标记准确率达79．1％，标记Sat_229、Satt335、Satt516和Sat_133抗病毒资源筛选的准确率均达70％以上，可以用作抗病毒分子辅助育种的选择标记。

英文摘要：

One hundred and ei coat mottling by the inoculation of ghty six soybean germplasms were screened for resistance to adult-plant and seed strain SMV1, and the coincident rate between adult-plant resistance, seed coat mottling resistance to SMV and linkage SSR markers were evaluated. The results showed that among these 186 germplasms,38 were resistant to both adult-plant and seed coat mottling;149 were only resistant to aduh-plant;45 were only resistant to seed coat mottling;and 26 were both susceptible to adult-plant and seed coat mottling. 6 SSR markers, Sat_229, Sat_317, Satt335, Satt160, Satt516 and Sat_309, related to SMV adult-plant resistance, were used to identify the 186 soybean germplasms. The coincident rates between the SSR alleles and the resistant presence were 68.9 % ,74.3 % , 71.1%, 69.8 % , 77.4 % and 68.2% , respectively ; 8 SSR markers, Sat_297, Sat_229, Sat_317, Satt335,Sct_188,Satt160, Satt516 and Sat_133 ,related to SMV seed coat mottling resistance,were used to identify the 186 soybean germplasms,the coincident rates of Sat_317 alleles with the resistant presence were 79.1%, and that of Sat_229, Satt335, Satt516 and Sat_133 were all over 70%. These markers related to SMV1 resistance were available for the marker assisted selection for SMV resistance breeding.