中文摘要：

目的探讨D-δ-三烯生育酚抗人乳腺癌MCF-7细胞的生物学活性。方法将细胞分为对照组（无药物培养）和实验组，实验组设低、中、高剂量组，分别加入终浓度为30，40，50μmol·L^-1的D-8-三烯生育酚，药物干预MCF-7细胞24h后，显微镜下观察各组细胞生长状态，四甲基偶氮唑盐（MTT）法、流式细胞仪分析细胞增殖、周期与凋亡。结果D-8-三烯生育酚抑制乳腺癌MCF-7细胞增殖，低、中、高剂量组细胞增殖抑制率分别为（9．33±1．68）％，（32．47±3．18）％和（76．63±1．00）％，与对照组比较，差异有统计学意义（P〈0．01）；低、中、高剂量组G。期细胞所占比例分别为（54．34±1．50）％，（58．24±0．81）％和（62．11±1．12）％，与对照组[（43．34±2．51）％]比较，差异有统计学意义（P〈0．05）；低、中、高剂量组贴壁细胞明显减少，漂浮死亡细胞明显增多，细胞早期凋亡率Q4分别为（10．87±0．50）％，（17．87±2．40）％和（25．83±3．76）％，与对照组[（1．93±0．25）％]比较，差异有统计学意义（P〈0．05）。结论D-8-三烯生育酚能抑制MCF-7细胞增殖，阻滞细胞于G1期，诱导细胞凋亡，具有抗乳腺癌MCF-7细胞的生物学活性。

英文摘要：

Objective To explore the biological activity of D-5- tocotrienols against human breast cancer MCF-7 cells. Methods The Breast cancer MCF-7 cell line was treated in vitro with O（control）, S0 （low）,40 （middle）, and 50 （high） μmol . L^-1 D-5-tocotrienols,respectively for 24 h. Cell growth was observed under the microscope. MTI＂ analysis and flow cytometry were applied to measure cell proliferation,cell cycle and apoptosis. Results D-8-tocotrienols inhibited the proliferation of MCF- 7 cell line. The cell proliferation inhibition rates of the low, middle, and high dose groups were（9.33± 1.68 ）%, （32.47±3.18）% and（76.63±1.00） %, respectively, compared with the control group. The differences were statistically significant （P〈0.01）. The percentage of G1 phase cells was（54.34±1.50） %, （58.24±0.81） % and（62.11±1.12） % ,respectively in the experimental groups, all significantly higher than （ 43.34 ±2.51 ） % in the control group （ P 〈 0.05 ）. The percentage of adherent cells was significantly reduced, and the floating dead cells significantly increased in the experimental groups ,The rates of early apoptosis were significantly increased by （ 10.87 ±0.50） %, （ 17.87±2.40） %, and （25.83 ±3.76 ） %, respectively, compared to that by （ 1.93± 0.25 ） % in the control group （P〈0.05）. Conclusion D-5-tocotrienols has anti-breast cancer activity by inhibiting the proliferation of MCF-7 cell line,leading to cell cycle arrest at G1 phase and inducing apoptosis.