中文摘要：

目的探讨微小RNA-155(miR-155)在扩张型心肌病(DCM)患者外周血单个核细胞(PBMC)中的表达变化及其与心功能的关系。方法选择DCM患者(观察组)及同期健康志愿者(对照组)各30例,观察组NYHA心功能Ⅱ级10例、Ⅲ级11例、Ⅳ级9例。采用Ficoll-Hypaque密度梯度离心法分离两组空腹静脉血中的PBMC,实时荧光定量PCR法检测PBMC中miR-155的相对表达量。比较观察组不同NYHA心功能分级者PBMC中miR-155的相对表达量,并分析其相关性。观察组同时行彩色多普勒检查,记录其左心室射血分数(LVEF)、左心室舒张末期内径(LVEDd),分析两者与miR-155表达的相关性。结果观察组miR-155相对表达量为0.50±0.24,对照组为1.00±0.23;两组比较,P<0.01。观察组NYHA心功能Ⅱ、Ⅲ、Ⅳ级者miR-155相对表达量分别为0.77±0.10、0.47±0.10、0.23±0.08,多组间及组内两两比较,P均<0.01。观察组LVEF为28%±10%,LVEDd为(70±7)mm。观察组miR-155相对表达量与NYHA心功能分级、LVEF、LVEDd均呈负相关(r分别为-0.94、-0.67、-0.53,P<0.05或<0.01)。结论 DCM患者PBMC中miR-155表达降低,与患者心功能受损的严重程度有关。

英文摘要：

Objective To observe the expression changes of microRNA-155 ( miR-155) in the peripheral blood mono-nuclear cells ( PBMC) of patients with dilated cardiomyopathy ( DCM) and to explore the relationship between miR-155 level and heart function .Methods Thirty patients with DCM ( observation group ) and 30 healthy volunteers ( control group) were selected.In the observation group , 10 cases were grade Ⅱof NYHA functional classification , 11 cases were grade Ⅲand 9 cases were grade Ⅳ.PBMC in the two groups were separated by Ficoll-Hypaque density gradient centrifuga-tion, and the relative expression of miR-155 was detected by real-time fluorescence quantitative PCR .The miR-155 levels were compared between the observation group and the control group and among DCM patients with different NYHA function -al classification.In the observation group, the left ventricular ejection fraction (LVEF) and left ventricular end diastolic diameter ( LVEDd ) were recorded by color Doppler ultrasound .The relationships between miR-155 levels and LVEF , LVEDd were analyzed in DCM patients .Results The relative expression levels of miR-155 in the observation group and control group were (0.50 ±0.24) and (1.00 ±0.23), which showed a significant difference (P<0.01).The relative expression levels of miR-155 in NYHA functional classification Ⅱ,Ⅲ,ⅣDCM patients were (0.77 ±0.10), (0.47 ± 0.10) and (0.23 ±0.08), respectively.There were significant differences between each two groups (all P<0.01).In the observation group , the LVEF was 28%±10%, and the LVEDd was (70 ±7) mm.The miR-155 level was negatively correlated with NYHA functional classification , LVEF and LVEDd in DCM patients ( r=-0.94, -0.67 and -0.53, P<0.05 or P<0.01).Conclusions The expression level of miR-155 is down-regulated in PBMC of DCM patients and is correlated with the severity of impaired cardiac function .