中文摘要：

为了深入了解耳蜗螺旋神经节（SG）神经元的基本电生理特点，本研究首先成功培养了急性分离的大鼠SG神经元，然后采用穿孔全细胞膜片钳记录技术观察了培养SG神经元的基本电生理特性。结果表明，可从培养的SG神经元记录到动作电位，且不同的SG神经元具有异质性的放电特征。上述结果提示穿孔全细胞膜片钳记录技术可用于体外培养耳蜗SG神经元的电生理特征研究，为开展听觉信息传导和调控的功能研究开辟了新途径。

英文摘要：

In order to further investigate the electrophysiological properties of cochlea spiral ganglion （SG） neurons, at first, the cell culture method for acutely dissociated SG neurons from SD rats （postnatal 0 -5 d old） was successfully established. Then, the basic electrophysiological properties of the cultured SG neurons were examined with perforated whole cell patch-clamp recording technique in the present study. The present results showed that action potentials could be recorded from the cultured SG neurons and different SG neurons exhibited a variety of firing characters about their action potentials. The present results suggest that： （1） Pedorated whole cell pateh-clamp recording method can be used for examining the electrophysiolngical properties of the cultured SG neurons; （2） For the functional analysis of the hearing information transformation and regulation, perforated whole cell patch-clamp recording method might create a new approaching pathway.