中文摘要：

用Suzuki偶合方法合成了一系列铱配合物为能量受体,侧链为羧酸基团的阴离子型水溶性聚芴.聚芴（P4,P5,P6）中铱配合物含量分别为0%,2%和5%,聚芴P6在水溶液中具有较好的荧光能量荧光转移（FRET）效率.还研究了p H变化对聚合物水溶液荧光光谱性能的影响,结果表明当p H〉8时,所含羧基以钠盐形式存在使聚合物具有较好的溶解性和较强的荧光强度,p H〈8时,羧基以COOH形式存在,使聚合物溶解度降低造成聚集,荧光发生淬灭.并且进一步研究了不同的蛋白质和多糖对铱配合物含量为5%的聚合物（P6）的荧光光谱变化,如加入带正电荷的溶菌酶后可以发生比较明显的FRET现象,加入中性的血红蛋白后荧光强度略有下降,而疏水作用较强的组蛋白和带负电荷肝素的加入可以增强P6的荧光强度减弱聚合物的FRET,因此聚合物P6可以作为蛋白质和多糖的光学检测器.

英文摘要：

A series of water-soluble carboxylated polyfluorenes containing iridium complex as the energy acceptor was synthesized via Suzuki coupling reaction.These anionic polymers（P4,P5 and P6） have 0,2 and5 molar percentage of iridium（Ⅲ） complex units in the polymer backbone,respectively.The polymer（P6）with 5 mol% iridium（Ⅲ） complex shows efficient intrinsic fluorescence resonance energy transfer（FRET） in aqueous solution.The photoluminescence（PL） spectra studies show that the polymers（P4 and P6） have higher fluorescence intensity at high p H and quenched in water at low p H.As p H decreases the carboxylate groups become more protonation,which leads to more aggregation of the polymer chains.The polymer（P6）fluorescence changes were studied with lysozyme,hemoglobin,histone and heparin in PBS buffer solutions.Addition of lysozyme to the P6 solution led to quenching of the fluorene segment and gradual increase in the iridium（Ⅲ） complex emission band.Addition of hemoglobin to the polymer solution led to weak quenching due to the metalloprotein but neutral charged.Addition of histone and heparin to the P6 solution induced increase in emission for fluorene segments,which was due to the hydrophobic nature and the repulsion interaction between negatively charged heparin and P6,respectively.