中文摘要：

目的：研究阳极化纳米管钛加载庆大霉素能否抑制细菌生长,促进成骨细胞的活性.方法：阳极氧化法获得阳极化纳米管钛（ANTTi）.共沉淀法在ANTTi表面加载庆大霉素（ANTTi-G）,测定庆大霉素的释放曲线.琼脂平板抑菌实验检测ANTTi-G的抑菌能力.通过检测成骨细胞在材料表面的黏附、总蛋白含量、增殖、ALP活性及钙结节来监测ANTTi-G对成骨细胞活性的影响.结果：共沉淀法加载的抗生素随着时间的延长逐渐释放.ANTTi-G及Ti-G抑菌效果明显强于ANTTi及Ti组.第7天时,ANTTi-G及ANTTi组成骨细胞的黏附、增殖、总蛋白含量、ALP活性明显高于Ti及Ti-G组（P〈0.05）.第14天时,ANTTi-G及ANTTi组成骨细胞的黏附、总蛋白含量、ALP活性明显高于Ti及Ti-G组（P〈0.05）.结论：与传统的Ti或者ANTTi相比,ANTTi-G有更好的抑菌效果.同样,与Ti或Ti-G相比,ANTTi-G与成骨细胞的的生物相容性更好.关节置换中,ANTTi-G可以作为一种潜在的预防局部感染的新材料应用于临床.

英文摘要：

AIM： To investigate whether gentamicin coating on anodized nanotubular titanium（ ANTTi-G） can inhibit bacterial growth and promote osteoblast activity. METHODS： Anodized nanotubular titanium（ ANTTi） was obtained by anodic oxidation.The gentamicin was then loaded onto ANTTi by co-precipitation,and the release profile of gentamicin was measured. The bacteriostatic ability of ANTTi-G was tested by antibacterial test of agar plate. The effect of ANTTi-G on osteoblast activity was measured by detecting the the adhesion,total protein content,proliferation,ALP activity and the number of calcium nodules of the osteoblasts.RESULTS： The antibiotics loaded by co-precipitation was gradually released over time. The antibacterial effect of ANTTi-G and Ti-G were significantly stronger than those of ANTTi and Ti group.On the 7 th day,the adhesion,proliferation,total protein content and ALP activity of osteoblast on the surface of ANTTi-G and ANTTi groups were significantly higher than those of the Ti and Ti-G groups（ P〈0. 05）. On the 14 th day,the adhesion,total protein content,ALP activity and number of calcium nodules of osteoblasts on the surface of ANTTi-G and ANTTi groups were significantly higher than those of Ti and Ti-G groups（ P〈0. 05）.CONCLUSION： ANTTi-G has a better antibacterial effect than ANTTi alone or conventional titanium implants. ANTTi also has more optimal biocompatibility compared with conventional titanium implant loaded with gentamicin. This study introduced something related to ANTTi-G,which can be used as a new kind of implant with the potential of preventing local infections for joint replacement surgeries.