中文摘要：

目的：探讨针灸治疗阿尔茨海默病（AD）的作用机制，为临床针灸防治AD提供实验依据。方法：采用Wistar大鼠，SPF级、雄性80只，体质量（200±20）g，随机分为正常组、假手术组、模型组和针灸治疗组，每组20只。运用大鼠海马注射Aβ1-42的模型复制方法复制模型。除针灸治疗组外其余各组相应处理后均常规饲养，针灸治疗组针灸“百会”和“肾俞”穴，每日1次，7天为一疗程，治疗2个疗程，疗程间休息1目。运用免疫组化和定量RT—PCR技术分别检测AD模型大鼠海马神经元线粒体中Aβ-结合性乙醇脱氢酶蛋白（ABAD）和细胞色素氧化酶Ⅳ（COXIV）的水平。结果：针灸能有效下调大鼠海马神经元线粒体中ABAD的水平，提高COXⅣ的活性，与模型组比较差异具有统计学意义（P〈0．01），与正常组和假手术组比较差异无统计学意义（P〉0．05）。提示针灸能有效抑制ABAD的过度表达、提高COXⅣ的活性，减少活性氧自由基（ROS）的漏出，改善线粒体能量代谢障碍，从而达到防治AD的目的。结论：针灸防治AD可能与其抑制海马神经元线粒体中ABAD的过度表达、提高COXⅣ的活性从而改善线粒体能量代谢有关。

英文摘要：

Objective To explore action mechanism of acupuncture and moxibustion for Alzheimer＇s disease （AD） to provide evidence for prevention and treatment with acupuncture and moxibustion on AD in clinic. Methods Eighty SPF-grade male Wistar rats, （200±20）g, were randomly divided into a normal group, a shamoperation group, a model group and a treatment group, 20 cases in each one. The model was duplicated with injection of Aβ1-42 in rats＇ hippocampus. Expect the treatment group, the rest groups were treated with regular feeding after respective intervention. The treatment group was treated with acupuncture and moxibustion at ＂Baihui＂ （GV 20） and ＂Shenshu＂ （BL 23）, once a day, seven days as a treatment course and totally for two courses. There was one day of interval between the courses. The immunohistochemistry and quantitative RT-PCR methods were applied to test level of All-binding alcohol dehydrogense （ABAD） and cytochrome oxidase IV （COX IV ） in hippocampal neurons mitochondria. Results Acupuncture and moxibustion could reduce effectively level of ABAD and improve activity of COX IV in hippocampal neurons mitochondria in the treatment group, which has statistical significance compared with that in the model group （P〈0.01） and no statistical significance compared with that in the normal group and sham-operation group （P〉0.05）. This indicated that acupuncture and moxibustion could effectively suppress overexpression of ABAD, improve activity of COX IV and reduce leak of reactive oxygen species, which could improve metabolic disturbance of mitochondria energy to achieve the goal of prevention and treatment of AD. Conclusion The prevention and treatment of AD with acupuncture and moxibustion could be related with suppressing overexpression of ABAD and improving activity of COX IV in hippocampal neurons mitochondria to improve mitochondria energy metabolism.