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十字型微通道制备粒径均一的纤维素层析介质

ISSN号：0438-1157
期刊名称：化工学报
时间：2013.2.15
页码：676-682
分类：TQ352.7[化学工程]
作者机构：[1]浙江大学化学工程与生物工程学系,化学工程联合国家重点实验室,浙江杭州310027, [2]浙江工业大学化学工程与材料学院,绿色化学合成技术国家重点实验室培育基地,浙江杭州310032
相关基金：国家自然科学基金项目（21276228,21036005）;高等学校博士学科点专项科研基金项目（201001011100）;浙江省自然科学基金项目（LR12806003）.
相关项目：抗体分离过程中的介质、方法和集成化研究

关键词：微流芯片, 纤维素, 微球, 离子液体, 离子交换介质, microfluidic chip , cellulose, microsphere, ionic liquid, ion-exchange resin

中文摘要：

为了满足层析介质对粒径单分散的要求，利用十字型微通道的聚焦效应，形成粒径均一的纤维素微液滴，经固化成纤维素微球，再偶联配基制成了纤维素层析介质。以离子液体1-乙基-3-甲基咪唑甲基磷酸直接溶解微晶纤维素为水相，葵花籽油为油相，考察了微通道内微液滴的形成条件，优化了纤维素浓度、分散剂浓度、油水两相流速等因素，得到粒径约100μm的纤维素微液滴，CV值小于0．2。微液滴固化再生，得到球形度良好的纤维素微球，湿真密度1．019g·ml^-1，孔度94．6％，体均粒径105．5μm；进一步偶联DEAE配基，制得了离子交换层析介质，离子交换容量为123．3μmol·g^-1,牛血清白蛋白的饱和吸附容量Qm达到220mg·g^-1，有效扩散系数Dc为1．8×10^-11m^2·s^-1，体现出较好的蛋白质吸附性能。

英文摘要：

To prepare the cellulose-based chromatography matrix with uniform size, a cross-flow microchannel chip was used to control the droplet size of cellulose solution and then the microdroplets were solidified to obtain the monodisperse cellulose beads. In the present work, ionic liquid, [EMIM] MP, was used to dissolve the microcrystalline cellulose as water phase and sunflower oil was used as oil phase. The effects of cellulose concentration, surfactant addition, flow rates of oil phase and water phase were investigated. The optimized preparation condition was 2% cellulose solution as water phase, flow rate of water phase of 6 μl· min^-1 and flow rate of oil phase of 200 μl· min^-1. The microdroplets of cellulose solution thus obtained could be about 100 μm with the variation coefficient less than 0.2. After solidification and regeneration, cellulose beads were obtained with good sphericity. Wet density of beads was 1. 019 g · ml^-1 , porosity was 94.6%, and mean size was about 105. 5 /,m. The cellulose beads were coupled with DEAE ligand and μmol · g^- 1. Static adsorption adsorption capacity for bovine used as weak anion exchange and adsorption kinetics were serum albumin could coefficient was 1.8×10 ^-11m^2 · S^-1, which showed pote reach ntial a resin. The ion exchange studied. The results sho 220 mg · g^- 1 and the pplication for protein sep capacity was 123.3 wed that saturated effective diffusion aratlon.