中文摘要：

目的 探讨单壁碳纳米管（SWCNTs）对小鼠纹状体和海马的氧化损伤作用.方法 将40只雄性ICR小鼠随机分成12.5 mg/kg的SWCNTs处理组和含体积分数为0.1%的T80生理盐水对照组,分别观察1、7、14和28 d 4个时间点,每组5只,采用尾静脉注射法连续染毒5 d.冰浴上取小鼠海马和纹状体组织,匀浆后取上清,用黄嘌呤氧化法测定超氧化物歧化酶（SOD）活力,根据单位时间内GSH减少的量测定谷胱甘肽过氧化物酶（GSH-Px）的活力,用硫代巴比妥酸法（TBA）法检测脂质过氧化物丙二醛（MDA）含量.结果 SWCNTs处理组小鼠纹状体和海马中SOD活力第1天下降,第7天时最低,后逐渐升高,SWCNTs处理组小鼠第7天时纹状体和海马中SOD活力与对照组的差异均有统计学意义（P＜0.05）.与对照组比较,SWCNTs处理组第7天时小鼠纹状体中GSH-Px活力明显降低,第14天时明显升高,差异均有统计学意义（P＜0.05）;SWCNTs处理组第7天和第14天时小鼠海马中GSH-Px活力均明显低于对照组,差异有统计学意义（P＜0.05）;至第28天时,GSH-Px活力均恢复至对照组水平.SWCNTs处理组小鼠纹状体和海马中MDA含量第1天时降低,第7、14天时升高后逐渐降低,第7和14天时SWCNTs处理组小鼠纹状体和海马中MDA含量明显高于对照组,差异有统计学意义（P＜0.05）.结论 在该研究条件下,SWCNTs可引起小鼠纹状体和海马中抗氧化酶活力降低和脂质过氧化物含量的升高.

英文摘要：

Objective To study the oxidative damage of SWCNTs in striaturn and hippocampi of mice.Methods Forty male ICR mice were divided into experiment group （12.5 mg/kg SWCNTs） and control group （saline containing 0.1% Tween80） randomly. Each group was subdivided into 1, 7, 14 and 28 days group, 5 mice in each subgroup, then treated with tail intravenous injection for 5 continuous days. The striatum and hippocampus were isolated on the ice bath and homogenized in saline. SOD, GSH-Px, and MDA in the supernatants were measured with xanthine oxidize, GSH consumption in enzymatic reaction and TBA methods.Results After exposure to 12.5 mg/kg SWCNTs for 5 d, SOD activity in striaturn and hippocampi decreased on 1st day and reached the minimum on 7th day, then increased gradually. The SOD activity in the SWCNTs treatment groups on 7th day were significantly decreased when compared to control （P〈0.05）. Comparison with control group, GSH-Px activity in striatum obviously decreased on 7th day then increased on 14th day, the difference between 7th day and 14th day was significantly （P〈0.05）. GHS-Px activity in the hippocampi in SWCNTs group on 7th day and 14th day was significantly lower than that in control group （P〈0.05）, then increased to the level of control group on 28th day. MDA contents of striaturn and hippocampi in SWCNTs group reduced on 1st day, then gradually increased on7th day and 14th day, then reduced, MDA contents on7th day and 14th day n SWCNTs group were significantly higher than that in control group （P〈0.05）. Conclusions The results of present study indicated that SWCNTs could decrease antioxidase activity and increase the Lipid peroxide in striaturn and hippocampi of mice.