中文摘要：

目的建立改良型DEN诱发大鼠肝癌模型，探讨肝细胞癌变过程中的超微结构变化特点。方法用1％DEN溶液喂养Wistar大鼠诱发肝癌，在诱癌过程中给予大鼠3周休息间歇期，对不同诱癌阶段大鼠肝脏的超微结构进行动态观察。结果16周后诱癌组大鼠成癌率为96％（24／25），不同时期肝细胞核、核仁及胞质内细胞器的病理形态学改变有一定规律，观察到肝细胞吞噬细胞现象。结论设立间歇期提高了大鼠诱癌末期的成活率，但没有延长DEN诱癌的进程。该模型是动态研究肝癌发生的理想动物模型。肝细胞在癌变过程中出现变性、异型、吞噬细胞、恶变成癌细胞等超微结构特征。

英文摘要：

Objective To establish modified DEN - induced primary hepatocellular carcinomas model and observated the pathological characteristic during the process of hepatocarcinogenesis. Methods 1‰ DEN was administrate to Wistar rats interruptedly, there were 3 weeks for rats to take a rest during the hepatocarcinogenesis and the ultrastructural morphology of liver tissues were studied by electron microscopy in the early and lately stage of hepatocarcinogenesis. Results 24 Wistar rats （96%） hepatocarcinoma model were set up successfully after 16 weeks. A series of injured alterations were found in the mitochondria and the nuclei of the hepatocytes during the hepatocarcinogenesis, we also observed that hepatocytes phagocytized other cells during the process of hepatocarcinogenesis. Conclusion The Intermission in DEN administer for hepatocarcinoma induction can elevate survival rate of rats in the end of hepatocarcinogenesis, and can not change the process of hepatocarcinoma. It is an ideal animal model for dynamically observe the pathologic process of its development. There are some alterated features of hepatocyte＇s ultrastructure, such as denaturation, heterotype, phagocytic cell and canceration during the hepatocarcinogenesis.