中文摘要：

小麦品系L224-3苗期和成株期对目前大多叶锈菌生理小种表现抗性,鉴定和定位其中抗叶锈病基因对培育持久抗叶锈品种十分重要。苗期利用L224-3及37携带已知抗叶锈病基因的对照品系接种15个中国小麦叶锈菌小种,同时对由L224-3和感病品种郑州5389杂交获得的F2：3群体进行抗叶锈性遗传分析,进一步利用SSR和STS标记对L224-3中的抗叶锈病基因进行分子定位。遗传分析结果显示,L224-3可能携带1对显性抗病基因,暂命名为LrL224,标记分析表明,LrL224与1BL上的4个SSR标记barc8、gwm582、wmc419、wmc694和1个STS标记（ω-secali/Glu-B3）紧密连锁,其中距离最近的2个SSR标记为barc8、gwm582,与LrL224的遗传距离分别为4.3,4.6 cM。LrL224位于1BL染色体上,鉴于之前对其他已知抗叶锈病基因的研究,其抗性反应均与此不同,推断其可能为1个新的抗叶锈病基因。

英文摘要：

Wheat line L224-3 showed high resistance to most of Puccinia triticina pathotypes in the seedling and adult stage in the field.Identify and map the leaf rust resistance gene（s） in L224-3 is very useful to breed wheat cultivars with durable resistance to leaf rust.L224-3 and 37 check lines with known leaf rust resistance genes were inoculated with 15 pathotypes at seedling stage to postulate gene in L224-3.F2：3 population from the cross L224-3/Zhengzhou 5389 were identified with pathotype FHDQ for genetic analysis of leaf rust resistance in L224-3.SSR and STS markers were used to map the gene in L224-3.L224-3 might contain a dominant resistance gene,temporarily designated LrL224.Totally four SSR markers（barc8,gwm582,wmc419,wmc694） and one STS marker（ω-secali/Glu-B3） on 1B were closely linked to LrL224.The two flanking SSR loci were barc8 and gwm582,with the genetic distances of 4.3 and 4.6 cM,respectively.LrL224 was located on 1BL,and it showed different seedling reaction with other genes on 1B.Therefore LrL224 is likely to be a new leaf rust resistance gene.