中文摘要：

目的：探讨miR-34a在食管癌上皮间质转化中的作用。方法：向食管癌细胞EC109转染miR-34a模拟物，以qRT-PCR检测效率。以Westernblot检测EMT相关蛋白E-cadherin和Vimentin的表达。结果：伴随miR-34a表达升高，E-cadherin相应升高，Vimentin则下降(P＜0.05)。结论：miR-34a可促进食管癌EMT的发生。

英文摘要：

Objective:To investigate the role of miR-34a in epithelial mesenchymal transition of esophageal carcinoma.Methods:MiR-34a analog was transfected into esophageal cancer cell line EC109,and the efficiency of qRT-PCR detection was detected.Western and blot were used to detect the expression of EMT related proteins E-cadherin and Vimentin.Results:With the increase of miR-34a expression,E-cadherin increased and Vimentin decreased(P<0.05).Conclusion:MiR-34a can promote the occurrence of EMT in esophageal carcinoma.