中文摘要：

以猪胃肠道和土壤作为产酶微生物的主要来源，分别选用植酸钙、磷酸纤维素和淀粉作为指示剂，从猪胃肠道和土壤中筛选出高活力植酸酶产生菌9株（5个细菌、4个真菌）、纤维素酶产生真菌7株及高活力淀粉酶产生细菌2株．选用不同的培养基进行固体和液体振荡培养．结果表明，1）植酸酶的产酶高峰出现在振荡培养的第2天（7．14U·mL^-1），真菌固体培养的植酸酶活力最高可达到12．42U·g^-1；2）纤维素酶的产酶高峰出现在振荡培养的第2—4天（8．49—9．55U·mL^-1），固体培养的纤维素酶活力最高可达到2185U·g^-1；3）淀粉酶的产酶高峰出现在振荡培养的第2天，达到4．68U·mL^-1．对于真菌生产植酸酶和纤维素酶而言，固体发酵要优于液体发酵，说明固体培养条件更适合这2种酶的生产．

英文摘要：

This research focused on isolating enzyme-producing microorganisms from pig gut and soil. 9 strains of microorganisms （5 bacteria and 4 fungi） for phytase production, 7 strains of fungi for cellulase production, and 2 strains of bacteria for amylase production were isolated and purified by using the different indicators of calcium phytate, phospho-cellulose and starch, respectively. The microorganisms were incubated by liquid and solid fermentation to determine enzyme activity. The fermentation results indicated that： 1 ） Phytase activity reached the peak of 7.14 U · mL^-1 on the 2nd day of liquid fermentation and 12.42 U · g^-1 on the 4th day of solid fermentation; 2 ） Cellulase activity reached the peak of 8.49- 9.55 U · mL^-1 on the 2nd- 4th day of liquid fermentation and 2 185 U · g^-1 on the 4th day of solid fermentation; 3 ） Amylase activity reached the peak of 4.68 U · mL^-1 on the 2nd day of liquid fermentation. From the above results, it was better for the fungi to produce phytase and cellulase by using solid fermentation than by using liquid fermentation.