中文摘要：

将牙鲆基因组DNA经限制性内切酶RsaⅠ和Bst UI双酶切后采用FIASCO法构建酶切片段基因组文库。共挑取269个克隆,177个为阳性克隆,阳性克隆率为65.79%。经测序后获得191条微卫星序列,其中完美型占74.35%;非完美型占14.66%;混合型占10.99%。用引物设计软件Primer Premier 5.0设计引物153对,挑选其中的50对合成并在32个野生牙鲆个体中进行扩增,共31个位点具有多态性,统计结果后使用POPGENE软件进行分析,平均等位基因个数为3.939 4,平均有效等位基因数为3.052 2,平均观测杂合度为0.650 1,平均期望杂合度为0.586 6,各引物的Hardy-Weinberg平衡指数在0.012 587～0.984 917变动。这些筛选出的多态性微卫星标记可应用于进一步的牙鲆遗传多样性分析、家系分析及遗传图谱的构建等工作中。

英文摘要：

Olive flounder（Paralichthys olivaceus）is one of economically important fish in China.It is distributed widely over the Bohai Sea,the Yellow Sea and the South Sea in China,North Korea,Japan and along the east coastline of Russia.As its natural resource is exhausting gradually,olive flounder has become an important cultured fish in China.Polymorphic available DNA markers are needed for its genetic research.Microsatellites recently have become an extremely popular marker in a wide variety of genetic investigations.In this research,genomic DNA was extracted from fin of olive flounder collected from one locality along the Chinese coast.The genomic DNA was digested by using both RsaⅠ and Bst UI polymerase.And then a microsatellite-enriched genomic library was constructed.By using（GA）13 as probes,177 positive clones were identified from 269 clones,and 191 microsatellites were found.Among the 191 microsatellites,74.35% were perfect,14.66% were imperfect and 10.99% were compound type.50 markers were assessed using 32 individuals.Of the 50 loci 31 were found to be polymorphic.The result showed that the number of alleles ranged from 2 to 9,with an average of 3.939 4 alleles per locus.The number of effective alleles per locus is 3.052 2.The values of observed and expected heterozygosity per locus is 0.650 1 and 0.586 6,respectively.The values of Hardy-Weinberg equilibrium ranged from 0.012 587 to 0.984 917.These markers will be useful for population structure assessment,molecular marker-assisted selection and genetic linkage map construction for this species.