中文摘要：

目的探讨巢蛋白（nestin）和缺氧诱导因子-1α（HIF-1α）在人脑胶质瘤组织中的表达及其在皮质瘤血管生成中所起的作用。方法应用免疫组化两步法检测53例不同级别胶质瘤和5例正常胎脑组织中nes—tin和HIF-1α的表达。结果nestin在低级别和高级别胶质瘤的平均光密度值（A）分别为0．2463±0．0602和0．6438±0．0901，差异有显著性（P〈0．05）；HIF-1α在低级别和高级别胶质瘤的平均光密度值（A）分别为0．1334±0．0298和0．2746±0．0904，差异有显著性（P〈0．05）。结论胶质瘤中，nestin在新生微血管内皮细胞和HIF-1α仅在肿瘤细胞的表达与胶质瘤的病理分级有关，级别越高表达越强，而且两者呈正相关，提示可将nestin和，HIF-1α作为一种判断胶质瘤新生血管增生、恶性程度的参考依据。

英文摘要：

Objective To investigate the expression of nestin and HIF -1α in human glioma and their roles in the formation of blood vessels. Methods Immunohistochemistry was used to detect the expression of nestin and HIF -1α in 53 cases of human gliomas of different grades and 5 cases of normal fetus brains. Results The average optical density （A） of nestin in low grade and high grade of gliomas was O. 246 3 ± 0. 060 2 and 0. 643 8 ± 0. 090 1, respectively, with significant difference between the two groups （ P 〈 0.05 ）. The average density （ A ） of HIF - -1α in low grade and high grade of gliomas was O. 133 4 ±0. 029 8 and O. 274 6 ±0. 090 4, respectively, with significant difference between the two groups （ P 〈 0.05 ）. Conclusion The expression of nestin in newborn vascular endothelial cells and the expression of HIF -1α in glioma cells are associated with the grades of gliomas. Expression of nestin and HIF -1α can be used to evaluate vascular proliferation and malignancy of gliomas.