中文摘要：

在 transcriptional 水平监视表示是为植物基因的功能的分析的第一必要的步。直接涉及早反应得到或组成仅仅所有基因的小部分的编码基因的蛋白质影响了由得到或。对的 Transcriptional 回答各种各样得到 ors 广泛地包括 Nicotiana 和 Arahidopsis thaliana 在不同植物被学习了；然而，相应数据不为非标题白菜是可得到的。处理这个问题，我们描述孤立植物在 HR 的早正式就职 / 实行起来调整的 EST 的减少性的基于图书馆的途径导致了由的抑制得到或从 Phytophthora boehmeriae 的 PB90。根据他们在对三个染色体数据库的强风搜索的通常认为的鉴定， 70 起来调整的基因被分类进 9 部分：主要‘ m etabolism' 或‘ e nergy' 生产的某方面；在 synthesi 的家的‘ p 老一套或在 fate' 的‘ p 老一套；细胞的通讯 / 信号转导机制；包括 Beclin， SPT1，和 SPT2 的房间命运；参予抄写的 HLA-B 和 AGO1；功能还得为是坚定的细胞的运输和假想蛋白质或蛋白质。象 Beclin， thioredoxin， HLA-B， MAP3K， SPT1， SPT2，和 AGO1 那样的七选择基因是起来调整的由 PB90 导致了，建议基因可以在被触发 PB90 的 HR 起一个重要作用。

英文摘要：

Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. Transcriptional responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana; however, corresponding data aren＇t available for non-heading Chinese cabbage. To address this problem, we describe a suppression subtractive library-based approach to isolate the plant＇s ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases, 70 up-regulated genes were classified into 9 parts： some aspect of primary ＇metabolism＇ or ＇energy＇ production; ＇protein synthesis＇ or ＇protein fate＇; cellular communication/signal transduction mechanism; cell fates including Beclin, SPT1, and SPT2; HLA-B and AGO1 which participate in transcription; cellular transport and hypothetical proteins or proteins for which a function has yet to be determined. Seven selected genes such as Beclin, thioredoxin, HLA-B, MAP3K, SPT1, SPT2, and AGO1 were up-regulated induced by PB90, suggesting that the genes may play an important role in PB90-triggered HR.