中文摘要：

目的探明LRP16的表达与化疗药物足叶乙甙所诱导的肿瘤细胞凋亡是否产生作用以及可能的分子机制。方法首先将抑制LRP16表达的小干扰RNA,control,siRNA,siRNA-374（抑制率90%）,siRNA-668（抑制率60%）,对照分别转染入Hela细胞内,并通过足叶乙甙处理的细胞而导致DNA损伤。之后用CCK-8检测细胞活力;用流式细胞技术测定抑制LRP16的表达对Hela细胞凋亡率的影响;通过RT-PCR检测抑制LRP16表达之后核转录因子-κB（nuclear factor-κB,NF-κB）凋亡相关的下游靶基因的mRNA水平。结果在足叶乙甙（100μmol）的作用下,抑制LRP16的表达能够明显抑制细胞的增长（P〈0.05）,并呈浓度依赖性,同时增加细胞的凋亡率（P〈0.05）。RT-PCR结果显示,下调LRP16的表达减弱NF-κB的转录活性,进而对其下游靶基因的水平进行调节。结论 LRP16在足叶乙甙导致的凋亡起重要作用,而且可能是通过下调NF-κB的活性来发挥化疗药物抵抗效应。我们的实验结果初步证实抑制LRP16增加Hela细胞对足叶乙甙的敏感性。

英文摘要：

Objective To study the effect of LRP16 expression on etoposide-induced apoptosis of tumor cells and its possible molecular mechanism.Methods Hela cells were transfected with siRNA,control siRNA,siRNA-374 with an inhibiting rate of 90% for LRP16 expression,and siRNA-668 with an inhibiting rate of 60% for LRP16 expression,and then treated with etoposide to induce DNA damage.Viability of Hela cells was assayed with CCK-8.Effect of inhibiting LRP16 expression on apoptosis of Hela cells was detected by flow cytometry.mRNA level in downstream target genes related with NF-κB was measured by RT-PCR after LRP16 expression was inhibited.Results After treated with etoposide（100μmol/L）,the inhibited LRP16 expression significantly increased the proliferation and apoptosis of Hela cells in a dose-dependent manner（P0.05）.RT-PCR showed that down-regulation of LRP16 expression decreased the transcription activity of NF-κB and the mRNA level in downstream target genes related with NF-κB.Conclusion LRP16 plays an important role in etoposide-induced apoptosis of Hela cells by down-regulating the activity of NF-κB.Our experiment proves that inhibiting LRP16 expression increases the sensitivity of Hela cells to etoposide.