中文摘要：

目的探讨生长停滞特异基因6（Gas6）、基质细胞衍生因子1（SDF-1）在糖尿病视网膜病变（DR）发病机制中的作用。方法收集我院就诊的2型糖尿病患者120例,并根据糖尿病分期分为糖尿病无视网膜病变组（NDR）、单纯型DR组（BDR）及增生型DR组（PDR）3组,每组40例;正常组我院体检的健康成年人40例;测定血中白细胞和中性粒细胞的数量,总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDLC）、低密度脂蛋白（LDL-C）的含量,酶联免疫吸附试验（ELISA）测定受试者血清中Gas6、SDF-1α和SDF-1β的水平。结果 PDR组血清中TC水平明显高于其余3组（P=0.023、0.041、0.046）;PDR组白细胞计数显著降低,与其余3组比较差异有统计学意义（P=0.016、0.021,0.019）,BDR组的中性粒细胞计数高于PDR组（P=0.028）,正常组和NDR组HDL-C水平高于PDR组（P=0.031）,4组的血清TG水平差异无统计学意义（P=0.42）;正常组血清Gas6水平明显高于3组糖尿病患者,差异有统计学意义（P=0.028）;NDR组、BDR组和PDR组患者血清SDF-1α水平高于正常组（P=0.02）,且BDR组和PDR组高于NDR组（P=0.04）;BDR组和PDR组血清SDF-1β水平明显高于正常组和NDR组（P=0.014）;DR患者血清Gas6与TC、TG水平均呈弱正相关（P〈0.05）;DR患者血清SDF-1β水平与白细胞的数量呈弱正相关（P〈0.05）。结论 Gas6可能是通过改变患者的脂质代谢状况而参与DR的发病过程;DR病变后,由于视网膜部位的缺血、缺氧诱导了SDF-1α、SDF-1β的高表达,参与视网膜血管的新生;Gas6、SDF-1与DR的发病机制有关。

英文摘要：

Objective To study on the role of growth arrest specific gene 6 （ Gas6）, strormal cell-derived factor 1 （ SDF-1 ） in the pathogenesis of diabetic retinopathy. Methods One hundred and twenty patients with type 2 diabetes mellitus were collected. They were divided into three groups according to the diabetic stages： without diabetic retinopathy （NDR）, background diabetic retinopathy （BDR） and proliferative diabetic retinopathy （PDR）. There were 40 cases in each group. The normal group included 40 healthy adults . The number of white blood cell and neutrophil determination, total cholesterol （TC）, triglyceride（TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein（ LDL- C ） were measured. The serum Gas6, SDF-1 o~ and SDF-1 [3 level were measured by ELISA. Results Serum TC level in PDR group was significantly higher than that in the other three groups （P = 0. 023, 0. 041, 0. 046）. PDR group had significant decrease of white blood cell count with statistically significant difference compared with the other three groups （P = 0.016, 0.021, 0.019）. BDR group had higher neutrophil count than PDR group （P = O. 028）. Normal group and NDR group had higher level of HDL-C than PDR group （P = 0. 031 ）. There was no statistical significance of the level of serum TG （P = O. 42） among four groups. The level of serum Gas6 of normal group was significantly higher than that of the other three groups with diabetic patients （P =0.028） ; The level of serum SDF-1 o~ in group NDR, group BDR and group PDR were higher than that in normal group （P = 0.02）, and that in BDR group and PDR group were higher than that in NDR group （P = 0.04）. The levels of serum SDF-1 13 in BDR group and PDR group were significantly higher than that in the normal group and the NDR group （P --0.014）. Gas6 showed a weakly positively correlated with serum level of TC and TG in patients with DR （ P 〈 0.05 ）. The level of serum SDF-1 β was weakly positively correlated w