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Development of A Real-Time PCR Assay for Plasmodiophora brassicae and Its Detection in Soil Samples
  • ISSN号:2095-3119
  • 期刊名称:Journal of Integrative Agriculture
  • 时间:2013
  • 页码:1799-1806
  • 分类:O657.33[理学—分析化学;理学—化学]
  • 作者机构:[1]中国农业科学院蔬菜花卉研究所,北京100081
  • 相关基金:国家自然科学基金项目(31201473),大宗蔬菜产业技术体系建设专项项目(CARS-25)和国家科技支撑计划项目(2012BAD19B06),农业部园艺作物生物学与种质创制重点实验室项目资助
  • 相关项目:基于红外光谱的黄瓜枯萎病快速检测基础研究
中文摘要:

应用傅里叶变换红外光谱技术对还未表现症状的大白菜根肿病病样进行早期快速检测。结果表明,健康与染病的根部样品谱图存在一定差异,染病的根部样品在红外光谱图中的1227,1143和1105cm^-1有很强的吸收峰,而健康根部样品的谱图没有,结合吸收峰的峰面积值变化,可以对大白菜根肿病进行早期快速检测。同时,以聚合酶链式反应(PCR)进行检测加以佐证。二者在接种5天后均可以检测到大白菜根部组织中的根肿菌。这表明傅里叶变换红外光谱具有灵敏性高、方便、快速等优点,可以在病害未显症时进行早期快速检测,为植物病害的早期诊断提供了新的思路及方法。

英文摘要:

Fourier transform infrared spectroscopy (FTIR) technique was applied in the early rapid detection of Plasmodiophora brassicae in root of Chinese cabbage while the symptom had not appeared. The Chinese cabbage root was inoculated with Plasmodiophora brassicae. Chinese cabbage root infected with Plasnmodiophora brassicae and uninfected root samples showed their difference in FTIR spectra. The absorption peaks at 1 227, 1 143 and 1 105 cm^-1 were only found in the infected root samples, and combined with the variation in the peak area at these absorption peaks they could be used for early rapid detection for clubroot of Chinese cabbage. In addition, the polyrnerase chain reaction was used to verify the veracity of Fourier transform infrared spectroscopy (FTIR) technique. The results show that the detection results are consistent with each other, and they could detect the Plasmodiophora brassicae 5 days after inoculation. Results clearly demonstrated that the PTIR technology is a highly sensi- tive, convenient and quick one for the early rapid detection of clubroot of Chinese cabbage, and provides a new thought and method for the early detection of plant disease.

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期刊信息
  • 《农业科学学报:英文版》
  • 主管单位:中国农业科学院
  • 主办单位:中国农业科学院
  • 主编:
  • 地址:北京中关村南大街12号
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82106280 82106281
  • 国际标准刊号:ISSN:2095-3119
  • 国内统一刊号:ISSN:10-1039/S
  • 邮发代号:2-851
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国科学引文索引(扩展库),美国生物科学数据库,英国动物学记录,英国食品科技文摘
  • 被引量:69