中文摘要：

目的 评价滤膜法、多管发酵法以及酶底物法对氯化消毒损伤大肠杆菌的检测效果。方法 以大肠杆菌E.coli K12为指示细菌,并利用氯化消毒技术制备消毒损伤细菌,分别采用修复培养法和GB/T 5750—2006《生活饮用水标准检测方法》推荐的滤膜法、多管发酵法以及酶底物法进行检测。结果 修复培养法和多管发酵法均检出消毒损伤大肠杆菌,而滤膜法均未检出消毒损伤大肠杆菌;对于酶底物法,消毒5 min时样品消毒损伤大肠杆菌的检出率为0%-47.50%,消毒20 min所有样品均未检出消毒损伤大肠杆菌。与修复培养法比较,滤膜法和酶底物法消毒损伤大肠杆菌的检出率均较低,差异均有统计学意义（P〈0.05）。结论 多管发酵法可有效检测氯化消毒损伤大肠杆菌。

英文摘要：

Objective To evaluate the results of different methods including membrane filter method, multiple tube fermentation method and enzyme substrate method for the simultaneous detection of chlorine-injured E.coli. Methods Using E.coli K12 as the indicator bacteria, which was subjected to chlorine disinfection to acquire disinfection-injured bacteria. The injured E.coli was detected with repair culture method, and simultaneously with membrane filter method, multiple tube fermentation method and enzyme substrate method,respectively,which is recommended in Standard Examination Methods for Drinking Water. Results The repair culture method and multiple tube fermentation method could detect the total quantity of the injured E.coli, however, membrane filter method was failed; For the enzyme substrate method, the detection rate of disinfection samples at 5 rain ranged in 0%-47.50%,while the samples at 20 min were not detected. The membrane filter method and enzyme substrate method showed a low detection rate compared with the repair culture method, and the differences were not statistically significant （P〈0.05）. Conclusion Multiple tube fermentation method can be used in the detection of injured E.coli subjected to chlorine disinfection.