中文摘要：

目的：通过观察艾灸对克罗恩病（CD）大鼠结肠黏膜血红素氧合酶-1（HO-1）和趋化因子单核细胞趋化蛋白-3（MCP-3）蛋白表达的影响,探讨艾灸治疗CD的肠黏膜免疫机制。方法：采用国际公认的Morris方法制备CD大鼠模型。将动物随机分为模型组、隔药灸组、温和灸组、烟条灸组和热水灸组,并与正常组大鼠作对照,除正常组和模型组不做治疗外,其他各组大鼠选取双侧天枢进行相应治疗。应用苏木精-伊红（HE）染色,光镜下观察结肠病理学变化;采用免疫组化方法,观察大鼠结肠黏膜HO-1、MCP-3蛋白的表达。结果：与正常组比较,模型组大鼠可见黏膜缺损,绒毛破坏或缺失,黏膜下层充血水肿,腺体破坏或消失,杯状细胞减少,溃疡形成,大鼠结肠黏膜组织HO-1、MCP-3蛋白表达阳性目标总面积和阳性目标积分光密度显著增高（均P〈0.01）。治疗后,与模型组比较,隔药灸组和温和灸组大鼠结肠黏膜有明显改善,主要表现为肠腺排列规则,溃疡表面由新生的上皮细胞覆盖,或肠壁溃疡由新生的肉芽组织代替,黏膜下水肿减轻,有少量炎性细胞浸润,大鼠结肠黏膜组织HO-1、MCP-3蛋白表达阳性目标总面积和阳性目标积分光密度降低（均P〈0.01）;与烟条灸组和热水灸组相比,隔药灸组及温和灸组大鼠结肠组织HO-1、MCP-3蛋白表达阳性目标总面积和阳性目标积分光密度均显著降低（均P〈0.01）。结论：隔药灸和温和灸能够明显改善CD大鼠结肠黏膜组织炎性反应;能够下调CD大鼠结肠黏膜HO-1和MCP-3蛋白的表达,这可能是艾灸治疗CD的肠黏膜免疫作用机制之一。

英文摘要：

Objective： To observe the effect of moxibustion therapy on heme oxygenase-1（HO-1） and monocyte chemoattractant protein-3（MCP-3） protein expressions in the colonic mucosa of rats with Crohn＇s disease（CD）, and to explore the intestinal mucosal immune mechanism of moxibustion therapy in treating CD. Methods： The CD rat model was established using the internationally accepted Morris method. The rats were randomly divided into a model group, a herbal cake-partitioned moxibustion group, a mild moxibustion group, a cigarette moxibustion group and a hot compress group, which were compared with the normal group. Except the normal group and the model group, rats in the other groups accepted different moxibustion therapies on bilateral Tianshu（ST 25）. Hematoxylin-eosin（HE） staining was conducted and the pathological changes of the colon were observed under light microscope; the expressions of HO-1 and MCP-3 protein in rat＇s colonic mucosa were determined by immunohisto-chemistry. Results： Compared with the normal group, rats in the model group showed mucosal defect, villus destruction or loss, submucosal congestion and edema, glandular destruction or disappearance, reduced goblet cells, ulcer formation, significantly increased positive target area and positive target integral optical density of HO-1 and MCP-3 protein expression（all P〈0.01）. After treatment, compared with the model group, colonic mucosa was significantly improved in the herbal cake-partitioned moxibustion group and the mild moxibustion group, which mainly showed that the intestinal glands were arranged regularly, ulcer surfaces were covered by the neoformative epitheliums, or intestinal ulcers were replaced by the nascent granulation tissue, and submucosal edema was alleviated, with a small amount of inflammatory cell infiltration. The total areas and the integral optical densities of the positive targets for rat＇s colonic mucosa HO-1 and MCP-3 protein expressions were decreased（all P〈0.01）. Compared with the cigar