中文摘要：

目的：研究人脑皮层锥体神经元Na＋电流的急性缺氧反应特征以及白藜芦醇（resveratrol，RES）对Na＋电流缺氧反应的影响。方法：采用全细胞膜片钳记录法，在人脑皮层脑片上记录锥体神经元对TTX敏感的电压依赖性Na＋电流，观察急性缺氧和白藜芦醇对Na＋电流幅值和激活性质的影响。结果：（1）急性缺氧使人脑皮层脑片上的锥体神经元Na＋电流呈现短暂的小幅增大后，出现长时程抑制（P〈0．05），并使Na＋电流的I-V曲线左移（向超极化方向漂移）。（2）AMPA受体阻断剂NBQX阻断了缺氧引起的Na＋电流短暂增大（P〈0．01），并加剧了Na＋电流的缺氧后抑制（P〈0．01）；GABA。受体阻断剂Bicuculline对Na＋电流的缺氧性增大和缺氧后抑制无显著性影响（P〉0．05）；二者对缺氧引起的Na＋电流I—V曲线左移均无显著影响。（3）50μmol/L白藜芦醇阻断了Na＋电流的缺氧性增大（P〈0．01），增强了Na＋电流的缺氧后抑制（P〈0．05）；100μmol／L白藜芦醇显著延迟了Na＋电流的缺氧性反应，使Na＋电流的缺氧性增大现象消失，并使Na＋电流的缺氧后抑制现象衰减（P〈0．05）。50μmol／L和100μmol／L白藜芦醇均使Na＋电流激活曲线右移，接近正常。结论：人脑皮层锥体神经元Na＋电流对急性缺氧的反应主要表现为长时程抑制；AMPA受体活动可影响Na＋通道对急性缺氧的反应。白藜芦醇对人脑皮层锥体神经元Na＋电流缺氧反应的调节作用与剂量有关，小剂量可模拟NBQX的作用，而大剂量可降低Na＋通道对低氧的敏感性。

英文摘要：

Objective ： To study the properties and effects of resveratrol （RES） on Na＋ currents of human cortical pyramidal neurons in response to acute hypoxia. Methods： Whole-cell patch-clamp technique was used to record the TTX-sensitive voltage-dependent Na＋ currents of pyramidal neurons in human cortical slices, in order to investigate the effects of acute hypoxia and resveratrol on Na ~ current amplitude and activation properties. Results： （ 1 ） Acute hypoxia induced a brief and slightly increase in Na ~ current of human cortical pyramidal neurons followed by a long-lasting inhibition in amplitude （P 〈 0.05）. I-V curve and the activation curve of Na＋ currents were shifted leftwards （in the negative direction）. （2） AMPA receptor antagonist NBQX blocked the hypoxic increase of Na＋ currents （P 〈 0.01 ）, and enhanced the posthypoxic inhibition of Na＋ currents （P 〈 0.01 ） ; GABAA receptor antagonist Bicuculline had no significant effect on the hypoxic alterations of Na ＋ currents （ P 〉 0.05 ）. Both NBQX and bicuculline did not affect the shifting of I-V curve and activation curve. （3） 50 μmol/L resveratrol blocked the hypoxic increase of Na＋ currents of human cortical neurons （P 〈 0.01 ）, and aggravated the posthypoxic inhibition of Na＋ currents （P 〈 0.05 ） ; 100 μmol/L resveratrol significantly delayed the responses of Na＋ currents to acute hypoxia and completely blocked the hypoxic increase of Na＋ currents and decreased the posthypoxic inhibition （ P 〈 0.05 ）. The I-V curve and the activation curve of Na＋ currents were shifted to right by either 50 μmol/L or 100 μmol/L, approximating normal level. Conclusion： Acute hypoxia mainly caused long-lasting inhibition in Na＋ currents of human cortical pyramidal neurons. AMPA receptor may affect the responses of Na＋ channel to acute hypoxia. The effects of resveratrol upon hypoxic responses of Na＋ currents of human cortical neurons may be concentration-dependent. Low concentratio