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中文摘要：
基于微流控二维电泳芯片（2D-EMC）的流路特点,建立等效电阻模型,以便给出各端电压的合理取值范围,成功实现微流控芯片二维电泳分离。经实验测定各微通道电阻,在各端电压合理取值范围内,通过电流测量调整（优化）电压,得到了一组优化的电压控制方案,在化学发光-2D-EMC系统中成功实现了精氨酸和甘氨酸衍生物的二维分离。本方法显著减小了实现微流控芯片二维电泳分离的实验次数。
英文摘要：
Based on the microflow characteristics of two-dimensional electrophoresis microchip（2D-EMC）,an equivalent resistance model of 2D-EMC was constructed to obtain the rational ranges of the voltages applied in each reservoir,and to implement the efficient 2D-EMC separations on microchips.With the experimental determinations of resistance of each micro-channel and the rational ranges of the applied voltages,additionally,through the current measurement-voltage adjustment,the optimal control scheme of the applied voltages was obtained and then the mixed samples（arginine and glycine derivatives） on chemiluminescence detection-2D-EMC were successfully separated.With this approach,the trial number of optimizing voltages was remarkably decreased for efficient 2D-EMC separations.
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