中文摘要：

作为湖泊沉积物有机质的重要组成部分，α-纤维素受到了古气候研究者的极大关注，其稳定同位素已成功应用于古气候、古环境重建。目前，纤维素稳定同位素研究受到限制的原因在于从湖泊沉积物提取纤维素非常困难，已经建立的纤维素提取实验流程复杂。在综合前人研究方法的基础上，通过反复实验，建立了一套有效的四阶段提取沉积物α-纤维素实验方法（5％NaOH碱洗、5％HCl酸洗、亚氯酸钠和冰醋酸漂白以及17．5％NaOH碱洗）。红外光谱分析结果显示与标准纤维素谱线图是一致的；为了进一步验证实验结果，与前人研究方法提取的纤维素的碳、氧同位素对比表明本实验方法是可靠的，且简便易行。

英文摘要：

α-cellulose, as an important component of organic matter from lake sediments, has been paid much attention by paleoclimate researchers, and especially its isotopes have been extensively utilized to reconstruct paleoclimate and paleoenvironment. Recently, the limited application of stable isotope of cellulose is attributed to difficulty to extract pure cellulose from lake sediments, and the established experimental procedure of cellulose extraction is very complicated. Based on repeated experiments, an effective four processes to extract cellulose is established, including alkaline-washing by 5 % NaOH, acid-washing by 5 % HCl, bleaching by glacial acetic acid and sodium chlorite and alkaline-washing by 17.5 % NaOH. The result of infrared spectrum shows that spectrogram is consistent with standard α-cellulose. Furthermore, in order to testify the result, compare with the pre-established method about stable carbon and oxygen isotope, which indicates this procedure is proved to be simple and feasible.