以‘泽香’葡萄扦插苗为试材,在水培条件下,研究了氯化镉(CdCl2)处理下葡萄根系和叶片内源一氧化氮(NO)、活性氧(ROS)的生成规律,以及根系丙二醛(MDA)含量与根系活力的变化.结果表明:在0~1 mmol·L^-1范围内,随着CdCl2处理浓度的增加,葡萄扦插苗根系和叶片NO含量、一氧化氮合酶(NOS)活性和根系活力先升高后下降;CdCl2浓度为0.01mmol·L^-1时,根系NO含量和NOS活性分别提高51%和63%,超过0.1 mmol·L^-1时则显著下降;CdCl2浓度为0.01和0.05 mmol·L^-1时,叶片NO含量和NOS活性显著提高.0.5和1.0 mmol·L^-1的CdCl2处理显著提高了根系O2^.-产生速率、H2O2含量和MDA含量;同浓度CdCl2处理下,叶片中O2^.-产生速率明显低于根系O2^.-,而H2O2含量明显高于根系.
Taking the cutting seedlings of grape variety‘Ze-xiang’as test materials,a hydroponic experiment was conducted to study the generation patterns of endogenous nitric oxide(NO) and reactive oxygen species(ROS) in their roots and leaves,and the changes of root MDA content and root activity under the stress of cadmium chloride(CdCl2).In the treatments 0-1 mmol·L^-1 of CdCl2,the NO content and NOS activity in roots and leaves and the root activity all presented a tendency of increasing first and decreasing afterwards with increasing CdCl2.At 0.01 mmol·L^-1 of CdCl2,the NO content and NOS activity in roots increased by 51% and 63%,respectively;but at 0.1 mmol·L^-1 of CdCl2,they decreased significantly.At 0.01 and 0.05 mmol·L^-1 of CdCl2,the leaf NO content and NOS activity had the greatest increase;at 0.5 and 1.0 mmol·L^-1 of CdCl2,the root O2^.-generation rate and H2O2 and MDA contents increased significantly.At the same concentrations of CdCl2,the O2^.-generation rate in leaves was much lower while the H2O2 content was much higher,compared with those in roots.