中文摘要：

通过将三（2-氨基乙基）胺（TREN）、6-羧基荧光素与溴乙酸苄酯结合而合成出多胺多羧型配体,并赋予化合物荧光成像的功能。进一步与顺磁性的六水合氯化钆（Ⅲ）反应获得兼具核磁共振成像（Magnetic Resonance Imaging,MRI）和光学成像功能的双模式核磁共振成像造影剂。通过核磁氢谱、碳谱、高分辨质谱和ICP-AES表征了配体和金属配合物的结构。研究结果表明,该配合物的纵向弛豫率（r1）为3.46mM^-1·s^-1,与小分子型商用造影剂如马根维显 （Gd-DTPA）接近。该配合物同时拥有66%的荧光量子产率,在紫外光照射下显示明亮的绿色荧光,为以后进行生物体内研究打下坚实基础。

英文摘要：

A fluorescent ligand with polydentate amino carboxylate moieties was synthesized from tris （2-aminoethyl）amine and benzyl 2-bromoacetate,followed by the introduction of fluorescent functionality through an amidation reaction between the primary amine group and 6-carboxy-fluorescein. After coordination with paramagnetic GdCl3 · 6H2O,a bimodal contrast agent combing the ability for magnetic resonance imaging （MRI） and optical imaging was formed. The structures of the ligands and the complexes were confirmed by both H- and C-NMR.high resolution mass spectrometry,and ICP-AES. This new MRI contrast agent showed a r1 value （longitudinal relaxivity） of 3.46 mM^-1 · s^-1, comparable to commercial small molecular contrast agent like Magevist （Gd-DTPA）. This complex also showed bright green fluorescence when shined with UV light, showing a high quantum yield of 66%, which is essential for clinical bimodal imaging.