中文摘要：

目的观察花青素对电离辐射致小鼠骨髓c-kit阳性细胞损伤的防护作用及可能的作用机制。方法经磁珠细胞分选法获得小鼠骨髓c-kit阳性细胞,分为对照组和花青素组,每组再分成3份,分别接受0、1及4 Gy的137Csγ射线照射。对照组加700μL细胞悬液及等体积的无血清造血干/祖细胞扩增培养基,花青素组加700μL细胞悬液及含2×10-5mol/L花青素的等体积无血清造血干/祖细胞扩增培养基（37℃,5%CO2）,照射前30 min加入,照射后继续培养18 h。采用化学发光法检测小鼠骨髓c-kit阳性细胞的细胞活力,以相对荧光强度（RLU）表示。甲基纤维素半固体培养法检测克隆形成数目（CFU-GM）。流式细胞术检测细胞内活性氧（ROS）及磷酸化组蛋白H2AX（γ-H2AX）平均荧光强度。结果组内比较：与0 Gy比较,经1 Gy和4 Gy照射后小鼠骨髓c-kit阳性细胞活力下降,CFU-GM下降,细胞内ROS增加,γ-H2AX平均荧光强度增加（P〈0.05）。组间比较：与1 Gy和4 Gy对照组比较,花青素1 Gy和4 Gy组骨髓c-kit阳性细胞活力增加,CFU-GM增加,细胞内ROS降低,DNA损伤程度（γ-H2AX平均荧光强度）降低（P〈0.05）。结论花青素通过降低细胞内ROS水平及DNA损伤程度起到对受照射小鼠骨髓c-kit阳性细胞的防护作用。

英文摘要：

Objective To observe the protective effect of anthocyanin on irradiation induced bone marrow c- kitpositive cell injury, and further explore its possible mechanism. Methods Mouse bone marrow c-kit positive cells werecollected by cell sorting method. There were 2 groups： control group and anthocyanin group, which were sub-divided intothree groups and received 0 Gy, 1 Gy and 4 Gy irradiation respectively. The control group was added 700 μL cell suspensionand an equal volume of serum-free hematopoietic stem/progenitor cell culture medium. The 2×10-5mol/L anthocyanin wasco-cultured with mouse bone marrow c-kit positive cells of anthocyanin group half an hour before irradiation exposure, thencells were cultured for 18 hours under the conventional culture conditions（37 ℃,5%CO2）. Mouse c-kit positive cell viabilitywas measured by bioluminescence, and which was reflected by relative light units（RLU）. The ability of colony-forming unitswas reflected by CFU-GM. The reactive oxygen species（ROS） level and mean fluorescence intensity（MFI） of γ-H2 AX weredetected by flow cytometry. Results Compared to un-irradiated control group, the cell viability and the number of CFU-GM were decreased significantly, while the ROS level and MFI of γ-H2 AX were increased in c-kit positive cells irradiatedwith 1 Gy and 4 Gy（P 〈 0.05）. Compared to 1 Gy and 4 Gy irradiation groups, c-kit positive cell viability and the number ofCFU- GM were increased, the ROS level and MFI of γ- H2 AX were decreased in anthocyanin group（P 〈 0.05）.Conclusion Anthocyanin exhibits a promising protective effect on radiation- induced bone marrow c- kit positive cellinjury, which may be related to the alleviating ROS and DNA damage in bone marrow cells.