中文摘要：

根据菜茵衣藻（Chlamydomonas reinhardtii）、团藻（Volvox carteri）、伞藻（Acetabularia cloiftonii）等生物的1，5-二磷酸核酮糖羧化酶／加氧酶（RuBisCO）的小亚基rbcS基因氨基酸的高度保守序列，设计一时简并引物．进行RT-PCR．209bp的PCR产物经测序分析及进行氨基酸序列同源性比对，表明克隆的序列为盐藻rbcS基因的cDNA片段．根据该序列信息．采用RACE（rapid amplification of cDNAends）的方法扩增其5’上游未知区和3’下游未知区．5’RACE得到的cDNA长度为约300bp，3’RACE得到的长度约380bp．三段序列拼接后cDNA全长为878bp，其中开放读码框包括190个氨基酸．此cDNA序列．推导成氨基酸序列与已知物种的rbcS基因相比对，同源性分别为V．carter／78％，Creinhardtii75％，A．cliftonii67％。据此可推断所克隆的序列为盐藻RuBisCO的小亚基cDNA序列．GenBank收录号为AY739272．

英文摘要：

Ribulose-1,5-bisphosphate carboxylase/oxygenase （Rubiscr） is a key enzyme in photosynthesis.A full-length cDNA encoding the rbcS of D.salina was cloned by the reverse transcription polymerase chain reaction （RT-PCR） and rapid amplification of cDNA ends （RACE） were reported. One pair of degenerate primer was designed according to conserved motifs of known rbcS from .Chlamydomonas reinhardtii, Volvox carteri,Acetabularia clifionii etc, and was used to amplify the rbcS cDNA fragment from the total RNAs of D.salina. The resulting cDNA was 209 base pairs in length and shared high identify with other known rbcS. According to the rbcS cDNA sequence,5＇ RACE and 3＇ RACE were performed to obtain the 5＇ upstreaming and 3＇ downstreaming sequences of the rbcS gene.The total length of the three fragments was 878 bp which contained an open reading frame encoding 190 amino acids. Homologous analysis indicated that the deduced amino acid sequences of rbcS had a high degree of identity with previously reported members of other rbcS gene. The nucleotide sequence data reported here had appeared in the GenBank database under accession number AY739272.